Anticorps Recombinant de lapin anti-VEGFR2/CD309

VEGFR2/CD309 Recombinant Antibody for WB, IHC, FC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

230238D7

N° de cat : 83049-4-RR

Synonymes

KDR, VEGFR2, VEGFR2/KDR, 230238D7, CD309

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Galerie de données de validation

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  WB analysis of human placenta using 83049-4-RR

  human placenta tissue was subjected to SDS PAGE followed by western blot with 83049-4-RR (VEGFR2/CD309 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 83049-4-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83049-4-RR (VEGFR2/CD309 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 83049-4-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83049-4-RR (VEGFR2/CD309 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 83049-4-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83049-4-RR (VEGFR2/CD309 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 83049-4-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83049-4-RR (VEGFR2/CD309 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human rectal cancer using 83049-4-RR

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 83049-4-RR (VEGFR2/CD309 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human rectal cancer using 83049-4-RR

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 83049-4-RR (VEGFR2/CD309 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HUVEC using 83049-4-RR

  1x10^6 HUVEC cells were surface stained with 0.25 ug VEGFR2/CD309 Recombinant antibody (83049-4-RR, Clone:230238D7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 83049-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83049-4-RR against Human KDR were performed. The affinity constant is 4.71 nM.

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Applications testées

• Résultats positifs en WB: tissu placentaire humain,
• Résultats positifs en IHC: tissu placentaire humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HUVEC,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

83049-4-RR cible VEGFR2/CD309 dans les applications de WB, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: VEGFR2/CD309 Protéine recombinante Eg0218
• Nom complet: kinase insert domain receptor (a type III receptor tyrosine kinase)
• Masse moléculaire calculée: 152 kDa
• Poids moléculaire observé: 150 kDa, 230 kDa
• Numéro d’acquisition GenBank: NM_002253
• Symbole du gène: KDR
• Identification du gène (NCBI): 3791
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KDR, also named as VEGFR-2, FLK1 and CD309, is a receptor for VEGF or VEGFC. KDR which belongs to the protein kinase superfamily, has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. KDR functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. Mutations of this gene are implicated in infantile capillary hemangiomas.

Publications

Species	Application	Title
human	WB	Cell Biol Toxicol; HNRNPA2B1 stabilizes NFATC3 levels to potentiate its combined actions with FOSL1 to mediate vasculogenic mimicry in GBM cells; Authors - Hanting Wang

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