Anticorps Recombinant de lapin anti-VEGFA

VEGFA Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242072G9

N° de cat : 84687-2-PBS

Synonymes

VEGF, 242072G9, L-VEGF, N-VEGF, Vascular Endothelial Growth Factor

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Galerie de données de validation

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  WB analysis of Recombinant protein using 84687-2-RR (same clone as 84687-2-PBS)

  Recombinant protein VEGF121 (Eg1263) was subjected to SDS PAGE followed by western blot with 84687-2-RR (VEGFA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84687-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01495-1

  Cytometric bead array standard curve of MP01495-1, VEGF Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84687-1-PBS. Detection antibody: 84687-2-PBS. Standard: Eg1263. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84687-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 84687-2-PBS against Human VEGF121 was performed. The affinity constant is 0.882 nM.

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  Affinity and Kinetic Characterization of 84687-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84687-2-RR against Human VEGFA were performed. The affinity constant is 92.0 pM.

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Informations sur le produit

84687-2-PBS cible VEGFA dans les applications de WB, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: VEGFA Protéine recombinante Eg1263
• Nom complet: vascular endothelial growth factor A
• Numéro d’acquisition GenBank: NM_001171628.1
• Symbole du gène: VEGFA
• Identification du gène (NCBI): 7422
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

VEGFA, also named VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38 kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ).

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