Anticorps Monoclonal anti-VEGFA

• Phare
• Validé par KD/KO

VEGFA Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E2H9

N° de cat : 66828-1-PBS

Synonymes

VEGF, Vascular endothelial growth factor A, long form, vascular endothelial growth factor A, Vascular Endothelial Growth Factor, N-VEGF

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Galerie de données de validation

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  WB analysis of MCF-7 using 66828-1-Ig (same clone as 66828-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 66828-1-Ig (same clone as 66828-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HUVEC using 66828-1-Ig (same clone as 66828-1-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NCI-H1299 using 66828-1-Ig (same clone as 66828-1-PBS)

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

• 

  WB analysis of T-47D using 66828-1-Ig (same clone as 66828-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MDA-MB-231 using 66828-1-Ig (same clone as 66828-1-PBS)

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HuH-7 using 66828-1-Ig (same clone as 66828-1-PBS)

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 66828-1-Ig (same clone as 66828-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66828-1-Ig (same clone as 66828-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66828-1-Ig (same clone as 66828-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66828-1-Ig (same clone as 66828-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66828-1-Ig (same clone as 66828-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66828-1-Ig (same clone as 66828-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66828-1-Ig (same clone as 66828-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66828-1-Ig (same clone as 66828-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66828-1-Ig (same clone as 66828-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66828-1-Ig (same clone as 66828-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66828-1-Ig (same clone as 66828-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human endometrial cancer using 66828-1-Ig (same clone as 66828-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human endometrial cancer tissue using VEGFA antibody (66828-1-Ig, Clone: 2E2H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human endometrial cancer using 66828-1-Ig (same clone as 66828-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human endometrial cancer tissue using VEGFA antibody (66828-1-Ig, Clone: 2E2H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human lung cancer using 66828-1-Ig (same clone as 66828-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using VEGFA antibody (66828-1-Ig, Clone: 2E2H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human lung cancer using 66828-1-Ig (same clone as 66828-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using VEGFA antibody (66828-1-Ig, Clone: 2E2H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 66828-1-Ig (same clone as 66828-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using VEGFA antibody (66828-1-Ig, Clone: 2E2H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 66828-1-Ig (same clone as 66828-1-PBS)

  1X10^6 MCF-7 cells were intracellularly stained with 0.5 ug Anti-Human VEGFA (66828-1-Ig, Clone:2E2H9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66828-1-PBS cible VEGFA dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: VEGFA Protéine recombinante Ag13500
• Nom complet: vascular endothelial growth factor A
• Masse moléculaire calculée: 412 aa, 46 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC065522
• Symbole du gène: VEGFA
• Identification du gène (NCBI): 7422
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ). This antibody can recognize all VEGFA isoforms.

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