Anticorps Recombinant de lapin anti-VEGFA
VEGFA Recombinant Antibody for WB, IF/ICC, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, souris
Applications
WB, IF/ICC, ELISA
Conjugaison
Non conjugué
CloneNo.
7H10
N° de cat : 81323-2-RR
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules MCF-7, cellules A431, cellules HUVEC, cellules MDA-MB-231, cellules RAW 264.7, cellules T-47D |
Résultats positifs en IF/ICC | cellules HeLa, cellules MCF-7 |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
WB | See 3 publications below |
IF | See 1 publications below |
Informations sur le produit
81323-2-RR cible VEGFA dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris
Réactivité | Humain, souris |
Réactivité citée | Humain, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Recombinant |
Type | Anticorps |
Immunogène | VEGFA Protéine recombinante Ag13500 |
Nom complet | vascular endothelial growth factor A |
Masse moléculaire calculée | 412 aa, 46 kDa |
Poids moléculaire observé | 40 kDa |
Numéro d’acquisition GenBank | BC065522 |
Symbole du gène | VEGFA |
Identification du gène (NCBI) | 7422 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par protéine A |
Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38 kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ).
Protocole
Product Specific Protocols | |
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WB protocol for VEGFA antibody 81323-2-RR | Download protocol |
IF protocol for VEGFA antibody 81323-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Oncol Rep Triple‑negative breast cancer cell‑derived piR‑31115 promotes the proliferation and migration of endothelial cells via METTL3‑mediated m6A modification of YAP1 | ||
Apoptosis CCDC134 enhances ovarian reserve function and angiogenesis by directly interacting with INHA in a mouse model of premature ovarian insufficiency | ||
Biomaterials Chiral nanoassembly remodels tumor microenvironment through non-oxygen-dependent depletion lactate for effective photodynamic immunotherapy |