Anticorps Recombinant de lapin anti-VEGFB

VEGFB Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242024C10

N° de cat : 84734-3-RR

Synonymes

242024C10, VEGF B, VEGF related factor, VEGF-B, VEGF-related factor

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Galerie de données de validation

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  WB analysis of Transfected HEK-293T using 84734-3-RR

  Human VEGFB Transfected HEK-293T cells were subjected to SDS PAGE followed by western blot with 84734-3-RR (VEGFB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of Transfected CHO-K1 using 84734-3-RR

  Immunofluorescent analysis of (4% PFA) fixed Transfected CHO-K1 cells using VEGFB antibody (84734-3-RR, Clone: 242024C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84734-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84734-3-RR against Human VEGFB were performed. The affinity constant is 4.28 nM.

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Applications testées

• Résultats positifs en WB: Human VEGFB Transfected HEK-293T cells,
• Résultats positifs en IF/ICC: Transfected CHO-K1,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84734-3-RR cible VEGFB dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: VEGFB Protéine recombinante Eg2458
• Nom complet: vascular endothelial growth factor B
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22-30 kDa
• Numéro d’acquisition GenBank: BC008818
• Symbole du gène: VEGFB
• Identification du gène (NCBI): 7423
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Vascular endothelial growth factor B (VEGFB) is the third vascular endothelial growth factor family member to be discovered in 1996 . It has been demonstrated that vascular endothelial growth factor B (VEGFB) plays a vital role in regulating vascular biological function. VEGFB binds to vascular endothelial growth factor receptor 1 (VEGFR1) and neuropilin 1 (NRP1), which activates a lot of downstream activators similar to most tyrosine kinase receptors such as p38 MAPK, ERK/MAPK, PKB/AKT, and PI3K.(PMID: 34948148, PMID: 37375853)

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