Anticorps Monoclonal anti-VEGF-C

VEGF-C Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

2B11F7

N° de cat : 67116-1-Ig

Synonymes

VEGFC, 2B11F7, vascular endothelial growth factor C, Vascular endothelial growth factor-related protein, VEGF C

---

Galerie de données de validation

• 

  WB analysis of MCF-7 using 67116-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67116-1-Ig (VEGFC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of T-47D using 67116-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 67116-1-Ig (VEGFC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 67116-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67116-1-Ig (VEGFC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCI-H1299 using 67116-1-Ig

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 67116-1-Ig (VEGFC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 67116-1-Ig

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 67116-1-Ig (VEGFC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of T-47D using 67116-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 67116-1-Ig (VEGFC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 67116-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 67116-1-Ig (VEGFC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 67116-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using VEGFC antibody (67116-1-Ig, Clone: 2B11F7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules A549, cellules HT-1080, cellules LNCaP, cellules NCI-H1299, cellules T-47D
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67116-1-Ig cible VEGF-C dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: VEGF-C Protéine recombinante Ag18182
• Nom complet: vascular endothelial growth factor C
• Masse moléculaire calculée: 419 aa, 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC035212
• Symbole du gène: VEGFC
• Identification du gène (NCBI): 7424
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VEGFC is a member of the platelet-derived growth factor / vascular endothelial growth factor (PDGF/VEGF) family. The main function of VEGFC is to promote the growth of lymphatic vessels (lymphangiogenesis). It acts on lymphatic endothelial cells (LECs) primarily via its receptor VEGFR-3 promoting survival, growth, and migration.

Publications

Species	Application	Title
human	WB	Cell Cycle; CircHGS enhances the progression of bladder cancer by regulating the miR-513a-5p/VEGFC axis and activating the AKT/mTOR signaling pathway; Authors - Yi Zhu

{{ptg:RelatedPrimaryAntibodies}}