Anticorps Polyclonal de lapin anti-VEZT

VEZT Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 17684-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 17684-1-AP (same clone as 17684-1-PBS)

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 17684-1-AP (VEZT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17684-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 17684-1-AP (same clone as 17684-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 17684-1-AP (VEZT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17684-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 17684-1-AP (same clone as 17684-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17684-1-AP (VEZT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17684-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 17684-1-AP (same clone as 17684-1-PBS)

  IP result of anti-VEZT (IP:17684-1-AP, 4ug; Detection:17684-1-AP 1:500) with mouse brain tissue lysate 4400ug. This data was developed using the same antibody clone with 17684-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 17684-1-AP (same clone as 17684-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17684-1-AP (VEZT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17684-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 17684-1-AP (same clone as 17684-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17684-1-AP (VEZT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17684-1-PBS in a different storage buffer formulation.

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Informations sur le produit

17684-1-PBS cible VEZT dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VEZT Protéine recombinante Ag11786
• Nom complet: vezatin, adherens junctions transmembrane protein
• Masse moléculaire calculée: 731 aa, 83 kDa
• Poids moléculaire observé: 89 kDa, 125 kDa
• Numéro d’acquisition GenBank: BC064939
• Symbole du gène: VEZT
• Identification du gène (NCBI): 55591
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Vezatin (VEZT) is an adherens junctions transmembrane protein which identified as a putative tumor suppressor. In case of Listeria infection, it promotes bacterial internalization by participating in myosin VIIa recruitment to the entry site. There're two isoforms with calculated MW 89 kDa and 65 kDa. With modification or glycosylation, the MW in Western blot detection will be presented as ~125 kDa and ~89 kDa (PMID: 11080149) or 65-71 kDa and 88-100 kDa (PMID: 20049712). There're some isoforms with MW less than 70 kDa.

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