Anticorps Polyclonal de lapin anti-SLC32A1/VGAT

SLC32A1/VGAT Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14471-1-PBS

Synonymes

SLC32A1, VGAT, GABA and glycine transporter, hVIAAT, Vesicular GABA transporter

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Galerie de données de validation

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  WB analysis of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

  unboiled mouse brain tissue were subjected to SDS PAGE followed by western blot with 14471-1-AP (VGAT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

  IP result of anti-VGAT (IP:14471-1-AP, 4ug; Detection:14471-1-AP 1:5000) with mouse brain tissue lysate 2100 ug. This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14471-1-AP (VGAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14471-1-AP (VGAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using SLC32A1/VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14471-1-PBS cible SLC32A1/VGAT dans les applications de WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC32A1/VGAT Protéine recombinante Ag5843
• Nom complet: solute carrier family 32 (GABA vesicular transporter), member 1
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC053582
• Symbole du gène: VGAT
• Identification du gène (NCBI): 140679
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SLC32A1, also known as VGAT (vesicular GABA transporter), functions in the uptake of GABA and glycine into synaptic vesicles. GABA (gamma-aminobutyric acid), is the major inhibitory neurotransmitter in the CNS. VGAT transports GABA and glycine into acidic vesicles and localizes to the synaptic vesicle in glycinergic and GABAergic neurons. And VGAT antibodies are useful markers for presynaptic GABAergic and glycinergic neurons.

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