Anticorps Polyclonal de lapin anti-VGLUT1

VGLUT1 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 55491-1-PBS

Synonymes

1, SLC17A7, BNPI, Brain-specific Na(+)-dependent inorganic phosphate cotransporter

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Galerie de données de validation

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  WB analysis using 55491-1-AP (same clone as 55491-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55491-1-AP (VGLUT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55491-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 55491-1-AP (same clone as 55491-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 55491-1-AP (VGLUT1 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55491-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 55491-1-AP (same clone as 55491-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 55491-1-AP (VGLUT1 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55491-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 55491-1-AP (same clone as 55491-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55491-1-AP (VGLUT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55491-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 55491-1-AP (same clone as 55491-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55491-1-AP (VGLUT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55491-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 55491-1-AP (same clone as 55491-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 55491-1-AP (VGLUT1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55491-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 55491-1-AP (same clone as 55491-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 55491-1-AP (VGLUT1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55491-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 55491-1-AP (same clone as 55491-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using VGLUT1 antibody (55491-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55491-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 55491-1-AP (same clone as 55491-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using VGLUT1 antibody (55491-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55491-1-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 55491-1-AP (same clone as 55491-1-PBS)

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human VGLUT1 (55491-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 55491-1-PBS in a different storage buffer formulation.

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Informations sur le produit

55491-1-PBS cible VGLUT1 dans les applications de WB, IHC, IF-P, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7
• Masse moléculaire calculée: 62 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: NM_020309
• Symbole du gène: VGLUT1
• Identification du gène (NCBI): 57030
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Vesicular glutamate transporter 1 (VGLUT1) is a critical protein involved in the packaging and release of the neurotransmitter glutamate in synaptic vesicles. VGLUT1 is predominantly expressed in neurons that release glutamate, the primary excitatory neurotransmitter in the central nervous system, it is a marker of glutamatergic neurons.

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