Anticorps Polyclonal de lapin anti-VGLUT2

VGLUT2 Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, IF-Fro, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29209-1-PBS

Synonymes

SLC17A6, Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, DNPI

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Galerie de données de validation

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  WB analysis using 29209-1-AP (same clone as 29209-1-PBS)

  unboiled mouse brain tissue were subjected to SDS PAGE followed by western blot with 29209-1-AP (VGLUT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29209-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 29209-1-AP (same clone as 29209-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29209-1-AP (VGLUT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29209-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 29209-1-AP (same clone as 29209-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29209-1-AP (VGLUT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29209-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 29209-1-AP (same clone as 29209-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using VGLUT2 antibody (29209-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29209-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 29209-1-AP (same clone as 29209-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using VGLUT2 antibody (29209-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29209-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 29209-1-AP (same clone as 29209-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using VGLUT2 antibody (29209-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29209-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 29209-1-AP (same clone as 29209-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using VGLUT2 antibody (29209-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 29209-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29209-1-PBS cible VGLUT2 dans les applications de WB, IHC, IF-P, IF-Fro, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VGLUT2 Protéine recombinante Ag29565
• Nom complet: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: NM_020346
• Symbole du gène: VGLUT2
• Identification du gène (NCBI): 57084
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

VGLUT2, also known as SLC17A6, belongs to the major facilitator superfamily. VGLUT2 is a multifunctional transporter that transports phosphate at the plasma membrane and glutamate in synaptic vesicles (PMID:33440152, 11432869). VGLUT2 is involved in neurotransmitter loading into synaptic vesicles (PMID: 11698620). VGLUT2 is predominantly expressed in adult and fetal brain, with highest expression in the medulla, substantia nigra, subthalamic nucleus, and thalamus, and low levels in the cerebellum and hippocampus (PMID:10820226).

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