Anticorps Polyclonal de lapin anti-Villin

• Phare
• Validé par KD/KO

Villin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(19)

N° de cat : 16488-1-AP

Synonymes

VIL1, D2S1471, VIL, villin 1

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Galerie de données de validation

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  WB analysis of mouse kidney using 16488-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16488-1-AP (Villin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16488-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16488-1-AP (Villin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 16488-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 16488-1-AP (Villin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 16488-1-AP

  IP result of anti-Villin (IP:16488-1-AP, 4ug; Detection:16488-1-AP 1:300) with mouse kidney tissue lysate 4000ug.

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  IHC staining of mouse small intestine using 16488-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16488-1-AP (Villin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 16488-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16488-1-AP (Villin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 16488-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16488-1-AP (Villin antibody at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 16488-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16488-1-AP (Villin antibody at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse small intestine using 16488-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using Villin antibody (16488-1-AP) at dilution of 1:200 and CoraLite®647-conjugated AffiniPure F(ab, CoraLite® Plus 488 PIGR antibody (CL488-22024, green), CoraLite® Plus 594 ZG16 antibody (CL594-67389, Clone: 1A7B9, red).

• 

  IF Staining of mouse small intestine using 16488-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using Villin antibody (16488-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse small intestine using 16488-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using Villin antibody (16488-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse small intestine using 16488-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse small intestine tissue using Villin antibody (16488-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of COLO 320 using 16488-1-AP

  Immunofluorescent analysis of COLO 320 cells using 16488-1-AP (Villin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 16488-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Villin (16488-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, tissu de côlon de souris, tissu hépatique de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu d'intestin grêle de souris, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'intestin grêle de souris,
• Résultats positifs en IF-Fro: tissu d'intestin grêle de souris,
• Résultats positifs en IF/ICC: cellules COLO 320,
• Résultats positifs en FC (Intra): cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 4 publications below
• IF: See 12 publications below

Informations sur le produit

16488-1-AP cible Villin dans les applications de WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Villin Protéine recombinante Ag9610
• Nom complet: villin 1
• Masse moléculaire calculée: 827aa,93 kDa; 826aa,93 kDa
• Poids moléculaire observé: 93 kDa
• Numéro d’acquisition GenBank: BC017303
• Symbole du gène: Villin
• Identification du gène (NCBI): 7429
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Villin 1 (VIL1) is a 95-kd F-actin bundling and severing protein and its expression is restricted to epithelial cells with a brush border, like epithelial cells of the intestinal mucosa, gall bladder, renal proximal tubules and ductuli efferentes of the testis. VIL1 has been reported to be an epithelial cell-specific anti-apoptotic protein, and to have an important function in regulating actin dynamics, cell morphology, epithelial-to-mesenchymal transitions, cell migration and cell survival. In addition, VIL1 is a useful diagnostic marker for of various cancer, like cervical and endometrial adenocarcinomas, renal cell carcinoma. VIL1 was recently identified as a novel biomarker predictive for postoperative recurrence and poorer prognosis of high serum AFP associated HCC.

Publications

Species	Application	Title
rat	IF	Sci Transl Med; Oral delivery of liquid mRNA therapeutics by an engineered capsule for treatment of preclinical intestinal disease; Authors - Xiangang Huang
human	IF	Proc Natl Acad Sci U S A; Organotypic models of type III interferon-mediated protection from Zika virus infections at the maternal-fetal interface.; Authors - Jacqueline Corry
mouse	IF	Clin Transl Med; NLRP3 inflammasome of renal tubular epithelial cells induces kidney injury in acute hemolytic transfusion reactions.; Authors - Zhixin Liu
human	WB	mBio; ASB3 expression aggravates inflammatory bowel disease by targeting TRAF6 protein stability and affecting the intestinal microbiota; Authors - Mingyang Cheng
pig	IF	PLoS Pathog; Bile acids promote the caveolae-associated entry of swine acute diarrhea syndrome coronavirus in porcine intestinal enteroids.; Authors - Qi-Yue Yang
mouse	WB	Front Cell Dev Biol; Cathelicidin-WA Protects Against LPS-Induced Gut Damage Through Enhancing Survival and Function of Intestinal Stem Cells.; Authors - Sisi Wang

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