Anticorps Monoclonal anti-Villin

Villin Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(11)

CloneNo.

2B7B9

N° de cat : 66096-1-Ig

Synonymes

VIL1, 2B7B9, D2S1471, VIL, villin 1

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Galerie de données de validation

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  WB analysis of human kidney using 66096-1-Ig

  human kidney tissue were subjected to SDS PAGE followed by western blot with 66096-1-Ig (Villin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 66096-1-Ig

  human colon tissue were subjected to SDS PAGE followed by western blot with 66096-1-Ig (Villin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human small intestine using 66096-1-Ig

  human small intestine tissue were subjected to SDS PAGE followed by western blot with 66096-1-Ig (Villin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 66096-1-Ig

  IP result of anti-Villin (IP:66096-1-Ig, 4ug; Detection:66096-1-Ig 1:1000) with mouse kidney tissue lysate 6000ug.

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  IHC staining of human colon using 66096-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66096-1-Ig (Villin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 66096-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66096-1-Ig (Villin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66096-1-Ig

  Immunofluorescent analysis of HepG2 cells using 66096-1-Ig (Villin antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

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Applications testées

• Résultats positifs en WB: Rein humain, tissu
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu de côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 4 publications below
• IF: See 6 publications below

Informations sur le produit

66096-1-Ig cible Villin dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Villin Protéine recombinante Ag9637
• Nom complet: villin 1
• Masse moléculaire calculée: 827aa,93 kDa; 826aa,93 kDa
• Poids moléculaire observé: 93-95 kDa
• Numéro d’acquisition GenBank: BC017303
• Symbole du gène: Villin
• Identification du gène (NCBI): 7429
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Villin 1 (VIL1) is a 95-kDa F-actin bundling and severing protein and its expression is restricted to epithelial cells with a brush border, like epithelial cells of the intestinal mucosa, gall bladder, renal proximal tubules and ductuli efferentes of the testis. VIL1 has been reported to be an epithelial cell-specific anti-apoptotic protein, and to have an important function in regulating actin dynamics, cell morphology, epithelial-to-mesenchymal transitions, cell migration and cell survival. In addition, VIL1 is a useful diagnostic marker for of various cancer, like cervical and endometrial adenocarcinomas, renal cell carcinoma. VIL1 was recently identified as a novel biomarker predictive for postoperative recurrence and poorer prognosis of high serum AFP associated HCC.

Publications

Species	Application	Title
mouse	IF	Gastroenterology; Stiffness Restricts the Stemness of the Intestinal Stem Cells and Skews Their Differentiation Towards Goblet Cells; Authors - Shijie He
mouse	IF	Theranostics; The Circadian Clock Gene Bmal1 Controls Intestinal Exporter MRP2 and Drug Disposition.; Authors - Fangjun Yu
mouse	IHC	Oxid Med Cell Longev; Sitagliptin Alleviates Radiation-Induced Intestinal Injury by Activating NRF2-Antioxidant Axis, Mitigating NLRP3 Inf--lammasome Activation, and Reversing Gut Microbiota Disorder.; Authors - Shanshan Huang
Human		Oncotarget; A three-protein signature and clinical outcome in esophageal squamous cell carcinoma.; Authors - Hui-Hui Cao
mouse,human	IF	Inflamm Bowel Dis; Excessive Mitochondrial Fission Suppresses Mucosal Repair by Impairing Butyrate Metabolism in Colonocytes; Authors - Shi-Chen Fu
human	IHC,IF	J Pharmacol Sci; Syntaxin 3 interacts with serotonin transporter and regulates its function.; Authors - Serika Motoike

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