Anticorps Polyclonal de lapin anti-Vimentin

• Phare
• Validé par KD/KO

Vimentin Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

N° de cat : 22031-1-AP

Synonymes

Cell and organelle markers, Cytoskeleton Marker, VIM, vimentin

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Galerie de données de validation

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  WB analysis of HEK-293 using 22031-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22031-1-AP (VIM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 22031-1-AP

  WB result of Vimentin antibody (22031-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Vimentin transfected A549 cells.

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  WB analysis of HeLa using 22031-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22031-1-AP (VIM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 22031-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 22031-1-AP (VIM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 22031-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 22031-1-AP (Vimentin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 22031-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22031-1-AP (Vimentin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 22031-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22031-1-AP (Vimentin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human kidney using 22031-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Vimentin antibody (22031-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human kidney using 22031-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Vimentin antibody (22031-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A549, cellules HeLa, tissu cardiaque de souris
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu rénal humain,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

22031-1-AP cible Vimentin dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Vimentin Protéine recombinante Ag16898
• Nom complet: vimentin
• Masse moléculaire calculée: 466 aa, 54 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC000163
• Symbole du gène: VIM
• Identification du gène (NCBI): 7431
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).