Anticorps Polyclonal de lapin anti-VIRMA/KIAA1429

• Phare
• Validé par KD/KO

VIRMA/KIAA1429 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30166-1-PBS

Synonymes

KIAA1429, VIRMA, MSTP054, virilizer

---

Galerie de données de validation

• 

  WB analysis using 30166-1-AP (same clone as 30166-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30166-1-AP (KIAA1429 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30166-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 30166-1-AP (same clone as 30166-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30166-1-AP (VIRMA/KIAA1429 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30166-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 30166-1-AP (same clone as 30166-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30166-1-AP (VIRMA/KIAA1429 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30166-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 30166-1-AP (same clone as 30166-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using VIRMA/KIAA1429 antibody (30166-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30166-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

30166-1-PBS cible VIRMA/KIAA1429 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VIRMA/KIAA1429 Protéine recombinante Ag32830
• Nom complet: KIAA1429
• Poids moléculaire observé: 200-210 kDa
• Numéro d’acquisition GenBank: BC003701
• Symbole du gène: KIAA1429
• Identification du gène (NCBI): 25962
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

VIRMA (KIAA1429) is a key component of m6A methyltransferase (writer) complex that include METTL3, METTL14, WTAP, VIRMA, HAKAI, ZC3H13, and RBM15. VIRMA mediates methylation in the 3'UTR and around the stop codon, thus affecting alternative polyadenylation. VIRMA has been reported to act as an oncogenic factor in breast cancer and liver cancer. KIAA1429 exhibits differential nuclear and cytoplasmic distribution in colorectal cancer samples, and high cytoplasmic expression of KIAA1429 is associated with poor response to chemotherapy. BRAF-mediated phosphorylation of KIAA1429 facilitates its aggregation in the cytoplasm. Furthermore, cytoplasmic KIAA1429 promotes WNT pathway activation by binding and stabilizing FZD7, thereby further enhancing cancer stemness and oxaliplatin resistance (PMID: 40611274). Moreover, this antibody can not recognize short isoforms.

{{ptg:RelatedPrimaryAntibodies}}