Anticorps Polyclonal de lapin anti-VISTA

VISTA Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 31619-1-PBS

Synonymes

C10orf54, B7-H5, Sisp-1, SISP1, PP2135

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Galerie de données de validation

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  WB analysis using 31619-1-AP (same clone as 31619-1-PBS)

  Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 31619-1-AP (VISTA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31619-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 31619-1-AP (same clone as 31619-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31619-1-AP (VISTA antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31619-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 31619-1-AP (same clone as 31619-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31619-1-AP (VISTA antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31619-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 31619-1-AP (same clone as 31619-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using VISTA antibody (31619-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31619-1-PBS in a different storage buffer formulation.

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Informations sur le produit

31619-1-PBS cible VISTA dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: chromosome 10 open reading frame 54
• Masse moléculaire calculée: 311 aa, 34 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC020568
• Symbole du gène: VISTA
• Identification du gène (NCBI): 64115
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H, and SISP1, is a member of the immunoglobulin superfamily. It serves not only as a positive regulator but also as a substrate of MT1-MMP and contributes at least in part to tumor invasion.

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