Anticorps Recombinant de lapin anti-VISTA

VISTA Recombinant Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

241003A4

N° de cat : 83919-4-PBS

Synonymes

C10orf54, SISP1, PP2135, GI24, B7-H5

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Galerie de données de validation

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  WB analysis of human peripheral blood platelets using 83919-4-RR (same clone as 83919-4-PBS)

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 83919-4-RR (VISTA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83919-4-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 83919-4-RR (same clone as 83919-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83919-4-RR (VISTA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83919-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 83919-4-RR (same clone as 83919-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83919-4-RR (VISTA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83919-4-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 83919-4-RR (same clone as 83919-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83919-4-RR (VISTA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83919-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 83919-4-RR (same clone as 83919-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83919-4-RR (VISTA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83919-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 83919-4-RR (same clone as 83919-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83919-4-RR (VISTA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83919-4-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 83919-4-RR (same clone as 83919-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using VISTA antibody (83919-4-RR, Clone: 241003A4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83919-4-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 83919-4-RR (same clone as 83919-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using VISTA antibody (83919-4-RR, Clone: 241003A4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83919-4-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 83919-4-RR (same clone as 83919-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using VISTA antibody (83919-4-RR, Clone: 241003A4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83919-4-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 83919-4-RR (same clone as 83919-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using VISTA antibody (83919-4-RR, Clone: 241003A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83919-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83919-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83919-4-RR against Human VISTA were performed. The affinity constant is 57.9 nM.

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Informations sur le produit

83919-4-PBS cible VISTA dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: chromosome 10 open reading frame 54
• Masse moléculaire calculée: 311 aa, 34 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC020568
• Symbole du gène: VISTA
• Identification du gène (NCBI): 64115
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H, and SISP1, is a member of the immunoglobulin superfamily. It serves not only as a positive regulator but also as a substrate of MT1-MMP and contributes at least in part to tumor invasion.

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