Anticorps Recombinant de lapin anti-VLDLR

VLDLR Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

242385H3

N° de cat : 85097-1-RR

Synonymes

Very low-density lipoprotein receptor, VLDL receptor, VLDL-R

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Galerie de données de validation

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  WB analysis using 85097-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85097-1-RR (VLDLR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of THP-1 using 85097-1-RR

  1x10^6 THP-1 cells were intracellularly stained with 0.25 ug VLDLR Recombinant Antibody (85097-1-RR, Clone:242385H3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules THP-1
• Résultats positifs en FC (Intra): cellules THP-1,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85097-1-RR cible VLDLR dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: very low density lipoprotein receptor
• Masse moléculaire calculée: 96 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: NM_003383
• Symbole du gène: VLDLR
• Identification du gène (NCBI): 7436
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The very low-density lipoprotein receptor (VLDLR) is a member of the LDL receptor family with a complex molecular weight profile due to post-translational modifications. The calculated molecular weight of the core protein is approximately 84-86 kDa, however, VLDLR undergoes extensive glycosylation during processing, resulting in higher apparent molecular weights observed in SDS-PAGE analyses. Multiple studies report that glycosylated VLDLR migrates as broad bands between 90-180 kDa under reducing conditions, with the most commonly observed forms at approximately 96 kDa (core glycosylated form), 115-135 kDa (mature fully glycosylated form), and 150-180 kDa (hyperglycosylated or dimeric forms).

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