Anticorps Recombinant de lapin anti-VPRBP

VPRBP Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241028B6

N° de cat : 83901-3-PBS

Synonymes

241028B6, DCAF1, DDB1- and CUL4-associated factor 1, EC:2.7.11.1, HIV 1 Vpr binding protein

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Galerie de données de validation

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  WB analysis using 83901-3-RR (same clone as 83901-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83901-3-RR (VPRBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83901-3-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83901-3-RR (same clone as 83901-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VPRBP antibody (83901-3-RR, Clone: 241028B6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83901-3-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83901-3-RR (same clone as 83901-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VPRBP antibody (83901-3-RR, Clone: 241028B6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83901-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00852-2

  Cytometric bead array standard curve of MP00852-2, VPRBP Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83901-3-PBS. Detection antibody: 83901-4-PBS. Standard: Ag2184. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 83901-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83901-3-RR against Human VPRBP were performed. The affinity constant is 0.538 nM.

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Informations sur le produit

83901-3-PBS cible VPRBP dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: VPRBP Protéine recombinante Ag2184
• Nom complet: Vpr (HIV-1) binding protein
• Masse moléculaire calculée: 1506 aa, 169 kDa
• Poids moléculaire observé: 169 kDa
• Numéro d’acquisition GenBank: BC022792
• Symbole du gène: VPRBP
• Identification du gène (NCBI): 9730
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

VprBP was first identified as a protein that can interact with HIV-1 viral protein R (PMID: 11223251). It is a component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex that could interact with HIV-1 virus Vpr protein and HIV-2 virus Vpx protein (PMID: 18332868; 17314515; 18606781). VprBP is a 1,507 amino acid protein that contains conserved domains, including YXXY repeats, the Lis homology motif, and WD40 repeats. Through binding to Vpr, VprBP allows Vpr to modulate the catalytic activity of the CUL4-DDB1 complex, which in turn leads to the induction of G2 phase arrest in the virus-infected cells (PMID: 17630831). Recently it has been reported that VprBP is able to regulate the p53-induced transcription and apoptotic pathway (PMID: 22184063).

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