Anticorps Polyclonal de lapin anti-VPS11

• Phare
• Validé par KD/KO

VPS11 Polyclonal Antibody for FC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 19140-1-AP

Synonymes

END1, hVPS11, PEP5, RING finger protein 108, RNF108, VPS11

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Galerie de données de validation

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  WB analysis using 19140-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19140-1-AP (VPS11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 19140-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19140-1-AP (VPS11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 19140-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19140-1-AP (VPS11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 19140-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 19140-1-AP (VPS11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 19140-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 19140-1-AP (VPS11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19140-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19140-1-AP (VPS11 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 19140-1-AP

  IP Result of anti-VPS11 (IP:19140-1-AP, 4ug; Detection:19140-1-AP 1:500) with HEK-293 cells lysate 1480ug.

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  FC experiment of HepG2 using 19140-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug VPS11 antibody (19140-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules K-562, tissu cardiaque humain, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain, tissu pancréatique de souris, tissu rénal humain
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

19140-1-AP cible VPS11 dans les applications de WB, IP, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VPS11 Protéine recombinante Ag6227
• Nom complet: vacuolar protein sorting 11 homolog (S. cerevisiae)
• Masse moléculaire calculée: 108 kDa
• Poids moléculaire observé: 108-112 kDa
• Numéro d’acquisition GenBank: BC065563
• Symbole du gène: VPS11
• Identification du gène (NCBI): 55823
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. In yeast, Vps proteins are involved in the trafficking of endocytic and biosynthetic proteins to the vacuole, which functionally resembles the lysosome of higher organisms. VPS11 is the human homolog of the yeast class C Vps11 protein, a subunit of HOPS (homotypic fusion and protein transport) complex. Mammalian Vps11 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.

Publications

Species	Application	Title
human	WB	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
human	WB,IF	J Cell Sci; Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L.; Authors - van der Kant Rik R ; KO Validated
human	WB	J Mol Biol; Proteomic and Biochemical Comparison of the Cellular Interaction Partners of Human VPS33A and VPS33B.; Authors - Morag R Hunter
human	WB, IF	J Biol Chem; Characterization of the mammalian CORVET and HOPS complexes and their modular restructuring for endosome specificity.; Authors - Rik van der Kant
human	IF,WB	J Cell Sci; The topology of lymphotoxin β receptor accumulated upon endolysosomal dysfunction dictates the NF-κB signaling outcome.; Authors - Magdalena Banach-Orłowska ; KD Validated
human	WB,IP	Biochem J; VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction.; Authors - Morag R Hunter