Anticorps Polyclonal de lapin anti-VPS16

• Phare
• Validé par KD/KO

VPS16 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

22

N° de cat : 17776-1-AP

Synonymes

hVPS16, VPS16

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Galerie de données de validation

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  WB analysis using 17776-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17776-1-AP (VPS16 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17776-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17776-1-AP (VPS16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17776-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17776-1-AP (VPS16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17776-1-AP

  IP Result of anti-VPS16 (IP:17776-1-AP, 3ug; Detection:17776-1-AP 1:500) with HeLa cells lysate 2480ug.

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  IHC staining of human liver using 17776-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17776-1-AP (VPS16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17776-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17776-1-AP (VPS16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17776-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17776-1-AP (VPS16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 17776-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17776-1-AP (VPS16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu hépatique humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 14 publications below
• IF: See 9 publications below

Informations sur le produit

17776-1-AP cible VPS16 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VPS16 Protéine recombinante Ag12202
• Nom complet: vacuolar protein sorting 16 homolog (S. cerevisiae)
• Masse moléculaire calculée: 839 aa, 95 kDa
• Poids moléculaire observé: 95 kDa
• Numéro d’acquisition GenBank: BC073959
• Symbole du gène: VPS16
• Identification du gène (NCBI): 64601
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. In yeast, Vps proteins are involved in the trafficking of endocytic and biosynthetic proteins to the vacuole, which functionally resembles the lysosome of higher organisms. Human VPS16 is the homolog of yeast class C Vps16 protein. VPS16 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.

Publications

Species	Application	Title
human	WB	Nat Commun; C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy; Authors - Shen Zhang
mouse	WB	Mol Cell; Distinct autophagosomal-lysosomal fusion mechanism revealed by thapsigargin-induced autophagy arrest.; Authors - Ian G Ganley ; KD Validated
mouse	WB	J Hazard Mater; VPS41-mediated incomplete autophagy aggravates cadmium-induced apoptosis in mouse hepatocytes; Authors - Tao Wang
human	WB	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
human	WB	Autophagy; Acetylation of STX17 (syntaxin 17) controls autophagosome maturation.; Authors - Qiuhong Shen
mouse	IF	Redox Biol; Regulation of TRPML1 channel activity and inflammatory exosome release by endogenously produced reactive oxygen species in mouse podocytes.; Authors - Guangbi Li