Anticorps Monoclonal anti-VPS18

VPS18 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E10G11

N° de cat : 67590-1-PBS

Synonymes

hVPS18, KIAA1475, PEP3, VPS18

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Galerie de données de validation

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  WB analysis using 67590-1-Ig (same clone as 67590-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67590-1-Ig (VPS18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67590-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67590-1-Ig (same clone as 67590-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67590-1-Ig (VPS18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67590-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67590-1-Ig (same clone as 67590-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67590-1-Ig (VPS18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67590-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 67590-1-Ig (same clone as 67590-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using VPS18 antibody (67590-1-Ig, Clone: 2E10G11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67590-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67590-1-PBS cible VPS18 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: VPS18 Protéine recombinante Ag29954
• Nom complet: vacuolar protein sorting 18 homolog (S. cerevisiae)
• Masse moléculaire calculée: 110 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC001513
• Symbole du gène: VPS18
• Identification du gène (NCBI): 57617
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Vps18 is a central member of Vps-C complex. Vps18 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.

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