Anticorps Polyclonal de lapin anti-VPS26A

• Phare
• Validé par KD/KO

VPS26A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(8)

Avis (2)

N° de cat : 12804-1-AP

Synonymes

VPS26, Vesicle protein sorting 26A, Vacuolar protein sorting-associated protein 26A, hVPS26

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Galerie de données de validation

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  WB analysis using 12804-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12804-1-AP (VPS26A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12804-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12804-1-AP (VPS26A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 12804-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12804-1-AP (VPS26A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12804-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12804-1-AP (VPS26A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 12804-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12804-1-AP (VPS26A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 12804-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12804-1-AP (VPS26A Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 12804-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12804-1-AP (VPS26A Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 12804-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 12804-1-AP (VPS26A antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL555-phalloidine stains F-actin (red).

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  IF Staining of HeLa using 12804-1-AP

  Immunofluorescent analysis of HeLa cells using 12804-1-AP (VPS26A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules HEK-293, tissu rénal de rat
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du col de l'utérus humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

12804-1-AP cible VPS26A dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VPS26A Protéine recombinante Ag3391
• Nom complet: vacuolar protein sorting 26 homolog A (S. pombe)
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC022505
• Symbole du gène: VPS26A
• Identification du gène (NCBI): 9559
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

In mammals, there are two paralogues of yeast Vps26, VPS26A and VPS26B (PMID: 16190980). VPS26 is a component of the retromer complex composed of VPS26 (VPS26A or VPS26B), VPS29, VPS35, SNX1, and SNX2. VPS26A and VPS26B subunits define distinct retromer complexes (PMID: 21920005). The retromer complex is important in recycling transmembrane receptors from endosomes to the trans-Golgi network (TGN).

Publications

Species	Application	Title
human	IF	Cell Metab; Phospholipase PLA2G6, a Parkinsonism-Associated Gene, Affects Vps26 and Vps35, Retromer Function, and Ceramide Levels, Similar to α-Synuclein Gain.; Authors - Guang Lin
human	WB	bioRxiv; Noncanonical roles of ATG5 and membrane atg8ylation in retromer assembly and function; Authors - Masroor Ahmad Paddar
human	WB	Elife; Noncanonical roles of ATG5 and membrane atg8ylation in retromer assembly and function; Authors - Masroor Ahmad Paddar
human	WB,IF	Sci Adv; Retromer promotes the lysosomal turnover of mtDNA; Authors - Parisa Kakanj
human	WB	Autophagy; RAB21 controls autophagy and cellular energy homeostasis by regulating retromer-mediated recycling of SLC2A1/GLUT1; Authors - Yifei Pei
human	WB,IHC	Int J Mol Sci; The Prognostic Value and the Oncogenic and Immunological Roles of Vacuolar Protein Sorting Associated Protein 26 A in Pancreatic Adenocarcinoma; Authors - Jihuan Hou ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xin (Verified Customer) (10-10-2022)

It is Ok to detect endogenous VPS26A although there are some non-specific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain lysate

VPS26A Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:12804-1-AP)

FH

Stephen (Verified Customer) (09-07-2019)

Oc-2 cells fixed with 4% PFA Perm. by 0.3% tx100 for 5 min blocked with 1% BSA in 1XPBS for 2 hours vps26a antibody incubated 1:300 overnight at 4 degrees in 1%BSA in 1x PBS. Co-stained with DAPI (blue) and Ph647 (red) to visualize DNA and F-actin respectively.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: mouse OC2 cells

VPS26A Antibody Immunofluorescence, validation (1:300 dilution) in mouse OC2 cells (Cat no:12804-1-AP)

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