Anticorps Recombinant de lapin anti-VPS28

VPS28 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241886D3

N° de cat : 84472-4-RR

Synonymes

Vacuolar protein sorting-associated protein 28 homolog, H-Vps28, ESCRT-I complex subunit VPS28, 241886D3

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Galerie de données de validation

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  WB analysis using 84472-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84472-4-RR (VPS28 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 84472-4-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using VPS28 antibody (84472-4-RR, Clone: 241886D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of SH-SY5Y using 84472-4-RR

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug VPS28 Recombinant antibody (84472-4-RR, Clone:241886D3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84472-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84472-4-RR against Human VPS28 were performed. The affinity constant is 1.72 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules Jurkat, cellules THP-1, cellules U-251
• Résultats positifs en IF/ICC: cellules A549,
• Résultats positifs en FC (Intra): cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84472-4-RR cible VPS28 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: VPS28 Protéine recombinante Ag7784
• Nom complet: vacuolar protein sorting 28 homolog (S. cerevisiae)
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC006485
• Symbole du gène: VPS28
• Identification du gène (NCBI): 51160
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VPS28 is a subunit of the ESCRT-I complex (endosomal sorting complex I required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. The ESCRT-I complex is composed of VPS23 (also known as TSG101), VPS28, VPS37, and MVB12A or MVB12B. VPS28 interacts with VPS23. Two alternative transcripts encoding different VPS28 isoforms have been described.

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