Anticorps Polyclonal de lapin anti-VPS33A

VPS33A Polyclonal Antibody for IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 16896-1-AP

Synonymes

hVPS33A, VPS33A

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Galerie de données de validation

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  WB analysis of human brain using 16896-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16896-1-AP (VPS33A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16896-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16896-1-AP (VPS33A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human cerebellum using 16896-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 16896-1-AP (VPS33A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 16896-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16896-1-AP (VPS33A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 16896-1-AP

  IP Result of anti-VPS33A (IP:16896-1-AP, 4ug; Detection:16896-1-AP 1:600) with HepG2 cells lysate 1600ug.

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules HepG2, cellules NIH/3T3, tissu de cervelet humain
• Résultats positifs en IP: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IF: See 2 publications below

Informations sur le produit

16896-1-AP cible VPS33A dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VPS33A Protéine recombinante Ag10422
• Nom complet: vacuolar protein sorting 33 homolog A (S. cerevisiae)
• Masse moléculaire calculée: 596 aa, 68 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC016617
• Symbole du gène: VPS33A
• Identification du gène (NCBI): 65082
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VPS33A is a Sec1/Munc18 family member and is a component of the homotypic fusion and protein sorting (HOPS)-tethering complex. The mammalian HOPS complex comprises six subunits (VPS11, VPS16, VPS18, VPS33A, VPS39, and VPS41) (PMID: 23901104). This complex is required for late endosome-lysosome and autophagosome-lysosome fusion (PMID: 24554770). VPS33A plays a role in vesicular transport to the lysosomal compartment.

Publications

Species	Application	Title
human	WB	Nat Commun; C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy; Authors - Shen Zhang
mouse	WB	J Hazard Mater; VPS41-mediated incomplete autophagy aggravates cadmium-induced apoptosis in mouse hepatocytes; Authors - Tao Wang
human	WB	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
human	WB	Autophagy; Acetylation of STX17 (syntaxin 17) controls autophagosome maturation.; Authors - Qiuhong Shen
human	WB	J Cell Biol; The Rab7 effector PLEKHM1 binds Arl8b to promote cargo traffic to lysosomes.; Authors - Rituraj Marwaha
human	IF	PLoS Pathog; Salmonella exploits the host endolysosomal tethering factor HOPS complex to promote its intravacuolar replication.; Authors - Aastha Sindhwani

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