Anticorps Polyclonal de lapin anti-VPS33B

VPS33B Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28653-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 28653-1-AP (same clone as 28653-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28653-1-AP (VPS33B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28653-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 28653-1-AP (same clone as 28653-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28653-1-AP (VPS33B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28653-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 28653-1-AP (same clone as 28653-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28653-1-AP (VPS33B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28653-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 28653-1-AP (same clone as 28653-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28653-1-AP (VPS33B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28653-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 28653-1-AP (same clone as 28653-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28653-1-AP (VPS33B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28653-1-PBS in a different storage buffer formulation.

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Informations sur le produit

28653-1-PBS cible VPS33B dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VPS33B Protéine recombinante Ag29886
• Nom complet: vacuolar protein sorting 33 homolog B (yeast)
• Masse moléculaire calculée: 617 aa, 71 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC016445
• Symbole du gène: VPS33B
• Identification du gène (NCBI): 26276
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

VPS33B, a homolog of yeast class C vacuolar protein sorting (vps) protein Vps33p, belongs to the STXBP/unc-18/SEC1 family. It may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. VPS33B mediates phagolysosomal fusion in macrophages (PMID: 18474358). Defects in VPS33B account for most cases of arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, which is a multisystem disorder associated with abnormalities in polarized liver and kidney cells (PMID: 20190753).

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