Anticorps Recombinant de lapin anti-VPS35

VPS35 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250521F1

N° de cat : 83328-2-RR

Synonymes

hVPS35, Maternal embryonic 3, Maternal-embryonic 3, MEM3, TCCCTA00141

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Galerie de données de validation

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  WB analysis using 83328-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83328-2-RR (VPS35 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HepG2, cellules NIH/3T3, cellules Raji, tissu rénal de rat, tissu rénal de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83328-2-RR cible VPS35 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: VPS35 Protéine recombinante Ag0333
• Nom complet: vacuolar protein sorting 35 homolog (S. cerevisiae)
• Masse moléculaire calculée: 92 kDa
• Poids moléculaire observé: 80-92 kDa
• Numéro d’acquisition GenBank: BC002414
• Symbole du gène: VPS35
• Identification du gène (NCBI): 55737
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VPS35 protein belongs to a group of vacuolar protein sorting (VPS) proteins, which ensure the proper delivery of organelle-specific proteins in eukaryotic cells. VPS35 is the core of a multimeric complex, termed the retromer complex, which is involved in retrograde transport of proteins from endosomes to the trans-Golgi network. Vps35 serves as the core of the multimeric complex by binding directly to Vps26 and Vps29 and SNX1. Northern blot analyses in 16 tissues showed that one transcript of Vps35 with a size of 3.6 kb was highly expressed in brain, heart, testis, ovary, small intestine, spleen, skeletal muscle, and placenta and expressed at moderate or low levels in other tissues. Another transcript of Vps35, a message of 3.0 kb, was also expressed with proportionally lower levels than the 3.6-kb transcript in all the tissues except that the 3.0-kb transcript was not detected in brain. Human Vps35 is mapped at 16q13-q21.

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