Anticorps Monoclonal anti-VPS37A

VPS37A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F9A4

N° de cat : 66327-1-PBS

Synonymes

ESCRT I complex subunit VPS37A, HCRP1, hVps37A, PQBP2, VPS37A

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Galerie de données de validation

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  WB analysis of SW 1990 using 66327-1-Ig (same clone as 66327-1-PBS)

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 66327-1-Ig (VPS37A Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66327-1-Ig (same clone as 66327-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66327-1-Ig (VPS37A Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66327-1-Ig (same clone as 66327-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66327-1-Ig (VPS37A Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66327-1-Ig (same clone as 66327-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66327-1-Ig (VPS37A Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66327-1-Ig (same clone as 66327-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66327-1-Ig (VPS37A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66327-1-Ig (same clone as 66327-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66327-1-Ig (VPS37A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66327-1-Ig (same clone as 66327-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66327-1-Ig (VPS37A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66327-1-PBS cible VPS37A dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: VPS37A Protéine recombinante Ag19108
• Nom complet: vacuolar protein sorting 37 homolog A (S. cerevisiae)
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: BC022363
• Symbole du gène: VPS37A
• Identification du gène (NCBI): 137492
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

VPS37A, also named as HCRP1, belongs to the VPS37 family. It is a regulator of vesicular trafficking process. VPS37A is a member of the endosomal sorting complex required for transport (ESCRT) system, in upper motor neuron disease. ESCRT has been shown to play a central role in intracellular trafficking, in the maturation of multivesicular bodies and the sorting of ubiquitinated membrane proteins into internal luminal vesicles.

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