Anticorps Polyclonal de lapin anti-VPS45

VPS45 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12006-1-PBS

Synonymes

---

Galerie de données de validation

• 

  IHC staining of mouse brain using 12006-1-AP (same clone as 12006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12006-1-AP (VPS45 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 12006-1-AP (same clone as 12006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12006-1-AP (VPS45 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 12006-1-AP (same clone as 12006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 12006-1-AP (VPS45 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 12006-1-AP (same clone as 12006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 12006-1-AP (VPS45 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 12006-1-AP (same clone as 12006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 12006-1-AP (VPS45 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 12006-1-AP (same clone as 12006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 12006-1-AP (VPS45 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 12006-1-AP (same clone as 12006-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12006-1-AP (VPS45 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 12006-1-AP (same clone as 12006-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using VPS45 antibody 12006-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

12006-1-PBS cible VPS45 dans les applications de IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VPS45 Protéine recombinante Ag2658
• Nom complet: vacuolar protein sorting 45 homolog (S. cerevisiae)
• Masse moléculaire calculée: 570 aa, 65 kDa
• Numéro d’acquisition GenBank: BC012932
• Symbole du gène: VPS45
• Identification du gène (NCBI): 11311
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The Sec1/Munc18 (SM) family of proteins regulate intracellular trafficking through interactions with individual SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) proteins and assembled SNARE complexes. The SM protein VPS45 (Vacuolar protein sorting-associated protein 45) has a calculated molecular mass of 65 kDa and shares 38% identity with yeast Vps45 which is required for the formation of yeast endosomal SNARE complexes and is thus essential for traffic through the endosomal system. VPS45 may play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network. (PMID: 8996080; 23166732)

{{ptg:RelatedPrimaryAntibodies}}