Anticorps Polyclonal de lapin anti-VPS4A

• Phare
• Validé par KD/KO

VPS4A Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14272-1-PBS

Synonymes

EC:3.6.4.6, hVPS4, Protein SKD2, SKD1, SKD2

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Galerie de données de validation

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  WB analysis using 14272-1-AP (same clone as 14272-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14272-1-PBS in a different storage buffer formulation.

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  WB analysis of U-937 using 14272-1-AP (same clone as 14272-1-PBS)

  WB result of VPS4A antibody (14272-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS4A transfected U-937 cells. This data was developed using the same antibody clone with 14272-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14272-1-AP (same clone as 14272-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14272-1-PBS in a different storage buffer formulation.

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  WB analysis of SMMC-7721 using 14272-1-AP (same clone as 14272-1-PBS)

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14272-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SMMC-7721 using 14272-1-AP (same clone as 14272-1-PBS)

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14272-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 14272-1-AP (same clone as 14272-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14272-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 14272-1-AP (same clone as 14272-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14272-1-AP (VPS4A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14272-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 14272-1-AP (same clone as 14272-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14272-1-AP (VPS4A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14272-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14272-1-PBS cible VPS4A dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VPS4A Protéine recombinante Ag5592
• Nom complet: vacuolar protein sorting 4 homolog A (S. cerevisiae)
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 48-55 kDa
• Numéro d’acquisition GenBank: BC047932
• Symbole du gène: VPS4A
• Identification du gène (NCBI): 27183
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Vacuolar Protein Sorting 4A (VPS4A) is one of two human homologs of the yeast protein Vps4 and member of the AAA protein family (ATPases associated with diverse cellular activities).

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