Anticorps Monoclonal anti-VPS4B

VPS4B Monoclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2H3G7

N° de cat : 68443-1-PBS

Synonymes

MIG1, Protein SKD1, SKD1, VPS4 2, VPS42, VPS4B

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Galerie de données de validation

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  WB analysis using 68443-1-Ig (same clone as 68443-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68443-1-Ig (VPS4B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68443-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 68443-1-Ig (same clone as 68443-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68443-1-Ig (VPS4B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68443-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 68443-1-Ig (same clone as 68443-1-PBS)

  IP result of anti-VPS4B (IP:68443-1-Ig, 4ug; Detection:68443-1-Ig 1:4000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 68443-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68443-1-Ig (same clone as 68443-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using VPS4B antibody (68443-1-Ig, Clone: 2H3G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68443-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68443-1-PBS cible VPS4B dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: VPS4B Protéine recombinante Ag11610
• Nom complet: vacuolar protein sorting 4 homolog B (S. cerevisiae)
• Masse moléculaire calculée: 444 aa, 49 kDa
• Poids moléculaire observé: ~50 kDa
• Numéro d’acquisition GenBank: BC039574
• Symbole du gène: VPS4B
• Identification du gène (NCBI): 9525
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

VPS4 (Vacuolar protein sorting-associated protein 4) is involved in intracellular protein trafficking, presumably at a late endosomal protein transport step. VPS4B is a ubiquitously expressed type I AAA+ (ATPases Associated with diverse cellular Activities) ATPase. It cooperates with its highly homologous paralog VPS4A (encoded by the VPS4A gene) to disassemble and release the Endosomal Sorting Complex Required for Transport (ESCRT) machinery. 68443-1-Ig specifically recognizes VPS4B. (PMID:31930723)

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