Anticorps Polyclonal de lapin anti-VPS54

• Phare
• Validé par KD/KO

VPS54 Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13327-1-PBS

Synonymes

Vacuolar protein sorting-associated protein 54, Tumor antigen SLP-8p, Tumor antigen HOM-HCC-8, Hepatocellular carcinoma protein 8, HCC8

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Galerie de données de validation

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  WB analysis of human brain using 13327-1-AP (same clone as 13327-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 13327-1-AP (VPS54 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13327-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 13327-1-AP (same clone as 13327-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13327-1-AP (VPS54 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13327-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 13327-1-AP (same clone as 13327-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 13327-1-AP (VPS54 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13327-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 13327-1-AP (same clone as 13327-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 13327-1-AP (VPS54 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13327-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 13327-1-AP (same clone as 13327-1-PBS)

  IP result of anti-VPS54 (IP:13327-1-AP, 4ug; Detection:13327-1-AP 1:300) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 13327-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13327-1-PBS cible VPS54 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VPS54 Protéine recombinante Ag4123
• Nom complet: vacuolar protein sorting 54 homolog (S. cerevisiae)
• Masse moléculaire calculée: 977 aa, 111 kDa
• Poids moléculaire observé: 111 kDa
• Numéro d’acquisition GenBank: BC030275
• Symbole du gène: VPS54
• Identification du gène (NCBI): 51542
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

VPS54 is a part of the Golgi-associated retrograde protein (GARP) complex protein required for tethering and fusion of endosome-derived transport vesicles to the trans-Golgi network. It may particpate in retrograde transport from early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD.

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