Anticorps Monoclonal anti-VPS54

VPS54 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3D12D1

N° de cat : 67404-1-PBS

Synonymes

Vacuolar protein sorting-associated protein 54, Tumor antigen SLP-8p, Tumor antigen HOM-HCC-8, Hepatocellular carcinoma protein 8, HCC8

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Galerie de données de validation

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  WB analysis of SH-SY5Y using 67404-1-Ig (same clone as 67404-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67404-1-Ig (VPS54 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67404-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 67404-1-Ig (same clone as 67404-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67404-1-Ig (VPS54 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67404-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67404-1-Ig (same clone as 67404-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67404-1-Ig (VPS54 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67404-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67404-1-Ig (same clone as 67404-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67404-1-Ig (VPS54 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67404-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51221-2

  Cytometric bead array standard curve of MP51221-2, VPS54 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67404-2-PBS. Detection antibody: 67404-1-PBS. Standard:Ag4123. Range: 0.195-25 ng/mL.

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  Cytometric bead array standard curve of MP51221-3

  Cytometric bead array standard curve of MP51221-3, VPS54 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67404-3-PBS. Detection antibody: 67404-1-PBS. Standard:Ag4123. Range: 0.195-12.5 ng/mL.

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Informations sur le produit

67404-1-PBS cible VPS54 dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: VPS54 Protéine recombinante Ag4123
• Nom complet: vacuolar protein sorting 54 homolog (S. cerevisiae)
• Masse moléculaire calculée: 977 aa, 111 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC030275
• Symbole du gène: VPS54
• Identification du gène (NCBI): 51542
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

VPS54 is a part of the Golgi-associated retrograde protein (GARP) complex protein required for tethering and fusion of endosome-derived transport vesicles to the trans-Golgi network. It may particpate in retrograde transport from early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD.

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