Anticorps Polyclonal de lapin anti-VILIP-1

VILIP-1 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 13919-1-AP

Synonymes

Hippocalcin like protein 3, HLP3, HPCAL3, HUVISL1, VILIP, VILIP 1, VILIP1, VILIP-1, visinin like 1, Visinin like protein 1, VISL1, VLP 1, VLP1, VLP-1, VSNL1

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Galerie de données de validation

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  WB analysis using 13919-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13919-1-AP (VILIP-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13919-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13919-1-AP (VILIP-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse tongue using 13919-1-AP

  mouse tongue tissue were subjected to SDS PAGE followed by western blot with 13919-1-AP (VILIP-1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13919-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13919-1-AP (VILIP-1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 13919-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 13919-1-AP (VILIP-1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13919-1-AP

  IP Result of anti-VILIP-1 (IP:13919-1-AP, 3ug; Detection:13919-1-AP 1:1000) with mouse brain tissue lysate 2640ug.

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  IF Staining of HeLa using 13919-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13919-1-AP (VILIP-1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HeLa, tissu cérébral de rat, tissu cérébral humain, tissu de cervelet de souris, tissu lingual de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

13919-1-AP cible VILIP-1 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VILIP-1 Protéine recombinante Ag4923
• Nom complet: visinin-like 1
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22-24 kDa
• Numéro d’acquisition GenBank: BC022012
• Symbole du gène: VSNL1
• Identification du gène (NCBI): 7447
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human, mouse	WB,IF	Free Radic Biol Med; Dimerization of visinin-like-protein 1 is regulated by oxidative stress and calcium and is a pathological hallmark of amyotrophic lateral sclerosis.; Authors - Martina P Liebl
mouse	WB	Front Cell Neurosci; Lack of Helios During Neural Development Induces Adult Schizophrenia-Like Behaviors Associated With Aberrant Levels of the TRIF-Recruiter Protein WDFY1.; Authors - Anna Sancho-Balsells