Anticorps Polyclonal de lapin anti-VTI1B

• Phare
• Validé par KD/KO

VTI1B Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14495-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 14495-1-AP (same clone as 14495-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14495-1-AP (VTI1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14495-1-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 14495-1-AP (same clone as 14495-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 14495-1-AP (VTI1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14495-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 14495-1-AP (same clone as 14495-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14495-1-AP (VTI1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14495-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 14495-1-AP (same clone as 14495-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 14495-1-AP (VTI1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14495-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 14495-1-AP (same clone as 14495-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14495-1-AP (VTI1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14495-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 14495-1-AP (same clone as 14495-1-PBS)

  IP result of anti-VTI1B (IP:14495-1-AP, 3ug; Detection:14495-1-AP 1:1000) with HeLa cells lysate 2500ug. This data was developed using the same antibody clone with 14495-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 14495-1-AP (same clone as 14495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14495-1-AP (VTI1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14495-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 14495-1-AP (same clone as 14495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14495-1-AP (VTI1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14495-1-PBS in a different storage buffer formulation.

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  IHC staining of human malignant melanoma using 14495-1-AP (same clone as 14495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma using 14495-1-AP (VTI1B antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14495-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human malignant melanoma using 14495-1-AP (same clone as 14495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma using 14495-1-AP (VTI1B antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14495-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14495-1-PBS cible VTI1B dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VTI1B Protéine recombinante Ag5906
• Nom complet: vesicle transport through interaction with t-SNAREs homolog 1B (yeast)
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC003142
• Symbole du gène: VTI1B
• Identification du gène (NCBI): 10490
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Fusion between membranes is mediated by specific SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) complexes. Two human SNARE proteins, VTI1A and VTI1B, are homologous to the yeast Q-SNARE Vtilp which is part of several SNARE complexes in different transport steps (PMID: 12067063). Both proteins had a distinct but overlapping localization. VTI1A is localized predominantly in the TGN, VTI1B in late endosomes (PMID:12067063; 21262811). VTI1B forms a SNARE complex with STX7, STX8 and VAMP8 which functions in the homotypic fusion of late endosomes. It is a component of the SNARE complex composed of STX7, STX8, VAMP7 and VIT1B that is required for heterotypic fusion of late endosomes with lysosomes. It has also been reported that VIT1B interacts with EpsinR, a protein involved in exocytic trafficking (PMID: 15371541).

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