Anticorps Polyclonal de lapin anti-Vitronectin

Vitronectin Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

14

Avis (1)

N° de cat : 15833-1-AP

Synonymes

S protein, Serum spreading factor, V75, vitronectin, VN, VNT, VTN

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Galerie de données de validation

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  WB analysis of human plasma using 15833-1-AP

  human serum was subjected to SDS PAGE followed by western blot with 15833-1-AP (Vitronectin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15833-1-AP

  rat plasma was subjected to SDS PAGE followed by western blot with 15833-1-AP (Vitronectin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 15833-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15833-1-AP (Vitronectin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 15833-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15833-1-AP (Vitronectin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 15833-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15833-1-AP (Vitronectin antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 15833-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15833-1-AP (Vitronectin antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 15833-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15833-1-AP (Vitronectin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 15833-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15833-1-AP (Vitronectin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 15833-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15833-1-AP (Vitronectin antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 15833-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15833-1-AP (Vitronectin antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of NIH/3T3 using 15833-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 15833-1-AP (Vitronectin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: rat plasma, cellules NIH/3T3, plasma humain, tissu testiculaire humain
• Résultats positifs en IHC: tissu hépatique humain, tissu de cirrhose hépatique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 5 publications below

Informations sur le produit

15833-1-AP cible Vitronectin dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, Plasmodium falciparum
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Vitronectin Protéine recombinante Ag8443
• Nom complet: vitronectin
• Masse moléculaire calculée: 478 aa, 54 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC005046
• Symbole du gène: VTN
• Identification du gène (NCBI): 7448
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Vitronectin is a 75-kDa glycoprotein present in blood and in the extracellular matrix (PMID: 10399314). Vitronectin is a multifunctional glycoprotein that mediates cell-to-substrate adhesion, inhibits the cytolytic action of the terminal complement cascade in vitro and binds to several serine protease inhibitors of the serpin family (PMID: 1372588).

Publications

Species	Application	Title
rat	IF,WB	Brain Pathol; Reconstituting neurovascular unit with primary neural stem cells and brain microvascular endothelial cells in three-dimensional matrix.; Authors - Hongjin Wang
human	WB,IF	Sci Rep; Molecular Camouflage of Plasmodium falciparum Merozoites by Binding of Host Vitronectin to P47 Fragment of SERA5.; Authors - Takahiro Tougan
human	WB,IF	J Cell Mol Med; Induction of migration of periodontal ligament cells by selective regulation of integrin subunits.; Authors - Mari Kawamura
human		Langmuir; Characterization of Initial Cell Adhesion on Charged Polymer Substrates in Serum-Containing and Serum-Free Media.; Authors - Takashi Hoshiba
human	WB	Proteomics; SILAC-based Quantitative Proteomic Analysis of Secretome between Activated and Reverted Hepatic Stellate Cells.; Authors - Hongyu Zhang
	WB	BMC Genomics; The fiber diameter traits of Tibetan cashmere goats are governed by the inherent differences in stress, hypoxic, and metabolic adaptations: an integrative study of proteome and transcriptome.; Authors - Bingru Zhao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jingwen (Verified Customer) (01-28-2020)

It worked well when I performed the western blot in cancer samples.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Cancer tissue

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