Anticorps Monoclonal anti-VWF

VWF Monoclonal Antibody for IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3F9F3

N° de cat : 66682-1-PBS

Synonymes

VWF, VWFpp, VWD, von Willebrand antigen II, F8VWF, 3F9F3

---

Galerie de données de validation

• 

  IHC staining of human breast cancer using 66682-1-Ig (same clone as 66682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66682-1-Ig (same clone as 66682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66682-1-Ig (same clone as 66682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66682-1-Ig (VWF antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66682-1-Ig (same clone as 66682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66682-1-Ig (VWF antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66682-1-Ig (same clone as 66682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66682-1-Ig (VWF antibody) at dilution of 1:2000 (under 10x lens). Proteolytic pre-treatment mediated antigen retrieval with . This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66682-1-Ig (same clone as 66682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66682-1-Ig (same clone as 66682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66682-1-Ig (same clone as 66682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66682-1-Ig (same clone as 66682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66682-1-Ig (same clone as 66682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66682-1-Ig (same clone as 66682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66682-1-Ig (same clone as 66682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66682-1-Ig (same clone as 66682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 66682-1-Ig (same clone as 66682-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human breast cancer tissue using VWF antibody (66682-1-Ig, Clone: 3F9F3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 66682-1-Ig (same clone as 66682-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human breast cancer tissue using VWF antibody (66682-1-Ig, Clone: 3F9F3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66682-1-PBS cible VWF dans les applications de IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: VWF Protéine recombinante Ag25578
• Nom complet: von Willebrand factor
• Symbole du gène: VWF
• Identification du gène (NCBI): 7450
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Von Willebrand factor (VWF) is a large multimeric glycoprotein found in blood plasma involved in hemostasis following vascular injury. Due to the multimeric nature of VWF, it can range in size from 500 to 20,000 kDa due to the differences in the number of subunits comprising the protein. Each subunit is approximately 250 kDa (PMID: 9759493). The biosynthesis of VWF in vivo is limited to endothelial cells (PMID: 4209883) and megakaryocytes (PMID: 2413071). VWF synthesized in endothelial cells is either released directly into the plasma via 27186a secretory pathway, or tubulized and stored in organelles unique to this cell type called Weibel-Palade bodies (PMID: 16459301). Whereas VWF synthesized in megakaryocytes is stored in the alpha granules of platelets (PMID: 2046403). The primary function of VWF is as an adhesive plasma glycoprotein, particularly factor VIII; an essential blood-clotting protein (PMID: 6982084). VWF is also important in platelet adhesion to wound sites by binding specifically to type I and type III collagen (PMID: 11098050), with larger VWF multimers being most effective (PMID: 24448155).

{{ptg:RelatedPrimaryAntibodies}}