Anticorps Monoclonal anti-ValRS

ValRS Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A2C3

N° de cat : 67935-1-PBS

Synonymes

G7A, Protein G7a, Valine tRNA ligase, ValRS, valyl tRNA synthetase, VARS, VARS1, VARS2

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Galerie de données de validation

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  WB analysis using 67935-1-Ig (same clone as 67935-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67935-1-Ig (ValRS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 67935-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 67935-1-Ig (same clone as 67935-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67935-1-Ig (ValRS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67935-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 67935-1-Ig (same clone as 67935-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67935-1-Ig (ValRS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67935-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67935-1-Ig (same clone as 67935-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67935-1-Ig (ValRS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67935-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67935-1-Ig (same clone as 67935-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ValRS antibody (67935-1-Ig, Clone: 1A2C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67935-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67935-1-PBS cible ValRS dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ValRS Protéine recombinante Ag9312
• Nom complet: valyl-tRNA synthetase
• Masse moléculaire calculée: 1264 aa, 140 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC012808
• Symbole du gène: ValRS
• Identification du gène (NCBI): 7407
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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