Anticorps Recombinant de lapin anti-VEGFA

VEGFA Recombinant Antibody for IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

2I18

N° de cat : 82864-7-RR

Synonymes

2I18, L-VEGF , N-VEGF, Vascular Endothelial Growth Factor, vascular endothelial growth factor A

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Galerie de données de validation

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  IHC staining of mouse lung using 82864-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 82864-7-RR (VEGFA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 82864-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 82864-7-RR (VEGFA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 82864-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82864-7-RR (VEGFA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 82864-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82864-7-RR (VEGFA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 82864-7-RR

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Vegfa antibody (82864-7-RR, Clone: 2I18 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 82864-7-RR

  Biolayer interferometry (BLl) kinetic assays of 82864-7-RR against Mouse Vegfa were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en IHC: tissu pulmonaire de souris, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules NIH/3T3,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82864-7-RR cible VEGFA dans les applications de IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: VEGFA Protéine recombinante Eg32035
• Nom complet: vascular endothelial growth factor A
• Numéro d’acquisition GenBank: NM_001287056
• Symbole du gène: Vegfa
• Identification du gène (NCBI): 22339
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Vegfa is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis.It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. It may play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis.

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