Anticorps Monoclonal anti-Villin

Villin Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B7B9

N° de cat : 66096-1-PBS

Synonymes

VIL1, 2B7B9, D2S1471, VIL, villin 1

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Galerie de données de validation

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  WB analysis of human kidney using 66096-1-Ig (same clone as 66096-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 66096-1-Ig (Villin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

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  WB analysis of human colon using 66096-1-Ig (same clone as 66096-1-PBS)

  human colon tissue were subjected to SDS PAGE followed by western blot with 66096-1-Ig (Villin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human small intestine using 66096-1-Ig (same clone as 66096-1-PBS)

  human small intestine tissue were subjected to SDS PAGE followed by western blot with 66096-1-Ig (Villin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 66096-1-Ig (same clone as 66096-1-PBS)

  IP result of anti-Villin (IP:66096-1-Ig, 4ug; Detection:66096-1-Ig 1:1000) with mouse kidney tissue lysate 6000ug. This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66096-1-Ig (same clone as 66096-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66096-1-Ig (Villin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66096-1-Ig (same clone as 66096-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66096-1-Ig (Villin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66096-1-Ig (same clone as 66096-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 66096-1-Ig (Villin antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66096-1-PBS cible Villin dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Villin Protéine recombinante Ag9637
• Nom complet: villin 1
• Masse moléculaire calculée: 827aa,93 kDa; 826aa,93 kDa
• Poids moléculaire observé: 93-95 kDa
• Numéro d’acquisition GenBank: BC017303
• Symbole du gène: Villin
• Identification du gène (NCBI): 7429
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Villin 1 (VIL1) is a 95-kDa F-actin bundling and severing protein and its expression is restricted to epithelial cells with a brush border, like epithelial cells of the intestinal mucosa, gall bladder, renal proximal tubules and ductuli efferentes of the testis. VIL1 has been reported to be an epithelial cell-specific anti-apoptotic protein, and to have an important function in regulating actin dynamics, cell morphology, epithelial-to-mesenchymal transitions, cell migration and cell survival. In addition, VIL1 is a useful diagnostic marker for of various cancer, like cervical and endometrial adenocarcinomas, renal cell carcinoma. VIL1 was recently identified as a novel biomarker predictive for postoperative recurrence and poorer prognosis of high serum AFP associated HCC.

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