Anticorps Monoclonal anti-Vimentin

• Phare
• Validé par KD/KO

Vimentin Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

195

CloneNo.

3H9D1

Avis (3)

N° de cat : 60330-1-Ig

Synonymes

VIM, Vimentin

---

Galerie de données de validation

• 

  WB analysis of Jurkat cells using 60330-1-Ig

  WB result of Vimentin antibody (60330-1-Ig, 1:4000) with si-Control and si-Vimentin transfected Jurkat cells.

• 

  WB analysis using 60330-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60330-1-Ig (Vimentin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  WB analysis of NIH/3T3 using 60330-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60330-1-Ig (Vimentin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 60330-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 60330-1-Ig (Vimentin Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 60330-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:102400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 60330-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60330-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:8000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 60330-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60330-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 60330-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 60330-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 60330-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 60330-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 60330-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60330-1-Ig (Vimentin Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 60330-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60330-1-Ig (Vimentin Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 60330-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 60330-1-Ig (Vimentin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HUVEC using 60330-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using Vimentin antibody (60330-1-Ig, Clone: 3H9D1 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of Retinal organoids using 60330-1-Ig

  Retinal organoids (day 250) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Vimentin with 60330-1-Ig at 1:500 dilution (green) and Caspase 3 using 19677-1-AP at 1:400 (red). Nuclear stain DAPI (blue). Scale bar = 20 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

• 

  FC experiment of Jurkat using 60330-1-Ig

  1X10^6 Jurkat cells were stained with 0.2 ug Anti-Human Vimentin (60330-1-Ig, Clone:3H9D1) and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1500 (red), or stained with 0.2 ug isotype control and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1500 (blue). Cells were fixed with 90% MeOH.

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules Jurkat, cellules NIH/3T3, cellules ROS1728, cellules U-251, cellules U2OS
• Résultats positifs en IHC: tissu d'appendicite humain, tissu d'amygdalite humain, tissu de cancer de l'endomètre humain, tissu de cancer du foie humain, tissu de cancer du sein humain, tissu de carcinome à cellules rénales humain, tissu de côlon humain, tissu de tumeur ovarienne humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HUVEC, cellules HepG2
• Résultats positifs en cytométrie: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochimie (IHC): IHC : 1:4000-1:16000
• Immunofluorescence (IF): IF : 1:500-1:2000
• Flow Cytometry (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 143 publications below
• IHC: See 23 publications below
• IF: See 71 publications below
• IP: See 2 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

60330-1-Ig cible Vimentin dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Vimentin Protéine recombinante Ag0489
• Nom complet: vimentin
• Masse moléculaire calculée: 466 aa, 54 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC000163
• Symbole du gène: VIM
• Identification du gène (NCBI): 7431
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.

Publications

Species	Application	Title
rat	IF	Adv Mater; Neonatal Tissue-derived Extracellular Vesicle Therapy (NEXT): A Potent Strategy for Precision Regenerative Medicine; Authors - Peng Lou
human	WB,IP, IF	Mol Cancer; CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer.; Authors - Ya Fan
mouse	WB	Acta Pharm Sin B; Celastrol induces ferroptosis in activated HSCs to ameliorate hepatic fibrosis via targeting peroxiredoxins and HO-1.; Authors - Piao Luo
human	WB	J Pineal Res; Melatonin and verteporfin synergistically suppress the growth and stemness of head and neck squamous cell carcinoma through the regulation of mitochondrial dynamics; Authors - Ye Young Shin
zebrafish	IF	Aging Cell; Müller Glia maintain their regenerative potential despite degeneration in the aged zebrafish retina.; Authors - Raquel R Martins
mouse	WB	Aging Cell; Lysyl oxidase-like 2 inhibitor rescues D-galactose-induced skeletal muscle fibrosis.; Authors - Yongxin Wu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Alessandro (Verified Customer) (12-09-2023)

good IF antibody. no unspecific staining

• Applications: Immunofluorescence
• Cell Tissue Type: Retinal organoids

FH

Aarushi (Verified Customer) (04-15-2023)

Primary antibody worked well after methanol treatment in a dilution of 1:200.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Tanycyte monolayer cell cultures (mouse)

Vimentin Antibody Immunofluorescence validation (1:200 dilution) in Tanycyte monolayer cell cultures (mouse) (Cat no:60330-1-Ig)

FH

Saba (Verified Customer) (03-03-2022)

The antibody worked perfectly!! The band was sharp and no unspecefic bands were seen.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF10A cells

Produits associés

Vimentin Polyclonal antibody

10366-1-AP

Desmin Polyclonal antibody

16520-1-AP

Desmin Monoclonal antibody

60226-1-Ig

Cytokeratin 8 Polyclonal antibody

17514-1-AP

GFAP Monoclonal antibody

60190-1-Ig

Nestin Monoclonal antibody

66259-1-Ig