Anticorps Monoclonal anti-Vimentin

Vimentin Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

IHC, IF, FC (Intra)

Conjugaison

CoraLite®488 Fluorescent Dye

Publications

1

CloneNo.

3H9D1

N° de cat : CL488-60330

Synonymes

VIM, vimentin

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Galerie de données de validation

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  IF Staining of HepG2 using CL488-60330

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®488 Vimentin antibody (CL488-60330, Clone: 3H9D1 ) at dilution of 1:400.

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  IF Staining of human tonsillitis using CL488-60330

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-60330 (Vimentin antibody) at dilution of 1:100..

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  IF Staining of human tonsillitis using CL488-60330

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-60330 (Vimentin antibody) at dilution of 1:100.

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  IF Staining of HepG2 using CL488-60330

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®488 Vimentin antibody (CL488-60330, Clone: 3H9D1 ) at dilution of 1:400.

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  FC experiment of Jurkat using CL488-60330

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®488 Anti-Human Vimentin (CL488-60330, Clone:3H9D1) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF: cellules HepG2, tissu d'amygdalite humain
• Résultats positifs en cytométrie: cellules Jurkat

Dilution recommandée

• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

CL488-60330 cible Vimentin dans les applications de IHC, IF, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Vimentin Protéine recombinante Ag0489
• Nom complet: vimentin
• Masse moléculaire calculée: 466 aa, 54 kDa
• Numéro d’acquisition GenBank: BC000163
• Symbole du gène: VIM
• Identification du gène (NCBI): 7431
• Conjugaison: CoraLite®488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 491 nm / 516 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Publications

Species	Application	Title
mouse	IHC, IF	J Mol Cell Biol; Cholesterol metabolic enzyme Ggpps regulates epicardium development and ventricular wall architecture integrity in mice.; Authors - Feng Zheng

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C