Anticorps Monoclonal anti-Vinculin

Vinculin Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris et plus (3)

Applications

WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA

Conjugaison

Non conjugué

Publications(203)

CloneNo.

2B5A7

Avis (15)

N° de cat : 66305-1-Ig

Synonymes

VCL, MVCL, MV, Metavinculin, CMD1W

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• Galerie de données de validation
• Proteintech vs. Other Vendors

• 

  WB analysis using 66305-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66305-1-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66305-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66305-1-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66305-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66305-1-Ig (Vinculin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig heart using 66305-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 66305-1-Ig (Vinculin Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 66305-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66305-1-Ig (Vinculin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 66305-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66305-1-Ig (Vinculin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66305-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66305-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66305-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66305-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66305-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66305-1-Ig(Vinculin antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HeLa using 66305-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Vinculin Monoclonal antibody (66305-1-Ig, Clone:2B5A7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules C6, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules HuH-7, cellules L02, cellules LNCaP, cellules MG U-87, cellules NIH/3T3, cellules U-251, tissu cardiaque de porc
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cardiaque de souris, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 162 publications below
• IHC: See 2 publications below
• IF: See 34 publications below
• CoIP: See 1 publications below

Informations sur le produit

66305-1-Ig cible Vinculin dans les applications de WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, Lapin, poisson-zèbre, porc, souris, Hamster
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Vinculin Protéine recombinante Ag24946
• Nom complet: vinculin
• Masse moléculaire calculée: 1133 aa, 124 kDa
• Poids moléculaire observé: 117 kDa
• Numéro d’acquisition GenBank: BC039174
• Symbole du gène: Vinculin
• Identification du gène (NCBI): 7414
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.

Publications

Species	Application	Title
mouse	WB	Crit Care; Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein.; Authors - Lingbing Zhang
human	WB	Adv Sci (Weinh); RBMS1 Coordinates with the m6 A Reader YTHDF1 to Promote NSCLC Metastasis through Stimulating S100P Translation; Authors - Yu Sun
human	WB	Cell Rep Med; Development of an orally bioavailable CDK12/13 degrader and induction of synthetic lethality with AKT pathway inhibition; Authors - Yu Chang
human	WB	Nat Commun; Modular assembly of the principal microtubule nucleator γ-TuRC.; Authors - Martin Würtz
human	WB	Redox Biol; Noscapine derivative 428 suppresses ferroptosis through targeting GPX4; Authors - Youping Zhang
human	WB	Dev Cell; HMOX1-LDHB interaction promotes ferroptosis by inducing mitochondrial dysfunction in foamy macrophages during advanced atherosclerosis; Authors - Xiang Peng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Monifa (Verified Customer) (08-31-2025)

I used vinculin as an alternative loading control to beta actin. After adjusting my transfer buffer to help vinculin transfer to my membrane, I can visualize the protein very well (with chemiluminescence).

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa

FH

Aditya (Verified Customer) (01-31-2025)

very clean bands, much better than the abcam antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

Morgane (Verified Customer) (01-09-2025)

Very good loading control with larger molecular size

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HCT116

Vinculin Antibody Western Blot validation (1:1000 dilution) in HCT116 (Cat no:66305-1-Ig)

FH

Daniel (Verified Customer) (10-24-2024)

The antibody works really well and it gives a very clean Western blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: eHAP, RPE1, HCT116 cells

FH

Lisa (Verified Customer) (04-29-2024)

Works super well!

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000

FH

Parijat (Verified Customer) (08-21-2023)

Works well as loading control

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293T

FH

Udesh (Verified Customer) (08-16-2023)

Worked well in WB at 1:3000 and IF at 1:100

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Bone Marrow MSCs

FH

Mohamad (Verified Customer) (07-03-2023)

Very good antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000

FH

Priya (Verified Customer) (01-17-2023)

I have used for human cardiomyocytes, mouse skin and liver tissues

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cardiomyoctyes, Keratinocytes, Liver and skin

FH

Macarena (Verified Customer) (10-07-2022)

excellent results.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: cell lines

FH

Jonas (Verified Customer) (07-29-2022)

This is my go to loading control stain to validate evenly loaded lanes Binding could be stronger but by using 1:500 dilution, a reliable staining can be achieved

• Applications: Western Blot
• Primary Antibody Dilution: 1:500

Vinculin Antibody Western Blot validation (1:500 dilution) in (Cat no:66305-1-Ig)

FH

Charlotte (Verified Customer) (07-26-2022)

Very good antibody. Very specific, super fast to reveal. Here we see Gli1 (160 kDa) because it is a mouse antibody too.

• Applications: Western Blot
• Primary Antibody Dilution: 1/5000
• Cell Tissue Type: NIH-3T3

Vinculin Antibody Western Blot validation (1/5000 dilution) in NIH-3T3 (Cat no:66305-1-Ig)

FH

AKIMASA (Verified Customer) (04-28-2021)

I could get the good quality band!

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: OVCAR8, PEO4, Kuramochi, OVSAHO, A2780, HEY,PEO1

FH

Chun (Verified Customer) (09-07-2020)

This is a fairly good antibody for immunoblotting.

• Applications: Western Blot
• Cell Tissue Type: HeLa

FH

Huai-Chin (Verified Customer) (06-08-2019)

Serving as a loading control, this antibody is not that good compare to other. Still work to some extent.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: 293T

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