Anticorps Monoclonal anti-Vinculin

Vinculin Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

3A11F8

Avis (3)

N° de cat : 66305-2-Ig

Synonymes

3A11F8, CMD1W, Metavinculin, MV, MVCL

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Galerie de données de validation

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  WB analysis using 66305-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66305-2-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66305-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66305-2-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66305-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66305-2-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary cancer using 66305-2-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66305-2-Ig (Vinculin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 66305-2-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66305-2-Ig (Vinculin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of hTERT-RPE1 using 66305-2-Ig

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using Vinculin antibody (66305-2-Ig, Clone: 3A11F8 ) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Applications testées

• Résultats positifs en WB: HGC-27 cells, cellules HeLa, cellules HUVEC, cellules K-562, cellules LNCaP, cellules MKN-45, plaquettes de sang périphérique humain, tissu cardiaque de lapin, tissu cardiaque de rat, tissu cardiaque de souris
• Résultats positifs en IHC: human ovary cancer tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules hTERT-RPE1,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

66305-2-Ig cible Vinculin dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, rat, souris

• Réactivité: Humain, Lapin, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Vinculin Protéine recombinante Ag24946
• Nom complet: vinculin
• Masse moléculaire calculée: 1133 aa, 124 kDa
• Poids moléculaire observé: 124 kDa
• Numéro d’acquisition GenBank: BC039174
• Symbole du gène: Vinculin
• Identification du gène (NCBI): 7414
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein that is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.

Publications

Species	Application	Title
	WB	Adv Sci (Weinh); RFWD3 Reprograms Nucleotide Metabolism Through PHGDH to Induce Chemoresistance In Osteosarcoma; Authors - Wenchao Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Matthieu (Verified Customer) (09-24-2025)

Band is very clear at the expected size

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: human fibroblasts

FH

Manon (Verified Customer) (09-24-2025)

Good housekeeping antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Fibroblasts

FH

Emilie (Verified Customer) (07-23-2025)

Used for Western blot on human fibroblasts and gives a clear, well-defined band. I recommend this product.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Fibroblasts

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