Anticorps Monoclonal anti-Vitamin D binding protein

Vitamin D binding protein Monoclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, FC, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

1E4D10

N° de cat : 66175-1-Ig

Synonymes

DBP, DBP/GC, GC, Gc globulin, VDB, VDBG, VDBP, VDBP,GC, Vitamin D binding protein

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Galerie de données de validation

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  WB analysis of human testis using 66175-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66175-1-Ig (Vitamin D binding protein antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  FC experiment of U-937 using 66175-1-Ig

  1X10^6 U-937 cells were intracellularly stained with 0.4 ug Anti-Human Vitamin D binding protein (66175-1-Ig, Clone:1E4D10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain
• Résultats positifs en cytométrie: cellules U-937,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

66175-1-Ig cible Vitamin D binding protein dans les applications de WB, IHC, IF, FC, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Vitamin D binding protein Protéine recombinante Ag9803
• Nom complet: group-specific component (vitamin D binding protein)
• Masse moléculaire calculée: 474 aa, 53 kDa
• Poids moléculaire observé: 52-58 kDa
• Numéro d’acquisition GenBank: BC057228
• Symbole du gène: GC
• Identification du gène (NCBI): 2638
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Vitamin D binding protein is a sparsely glycosylated serum protein responsible for highly specific binding and tissue-specific delivery of vitamin D and its metabolites. In addition, it is also an actin scavenger, and is the precursor to the immunomodulatory protein, Gc-MAF. Vitamin D binding protein has been proposed to have significant roles in C5a chemotaxis, osteoclast development and possibly in macrophage activation/recruitment.

Publications

Species	Application	Title
mouse	IHC,IF,WB,CoIP,ChIP	Theranostics; Vitamin D binding protein (VDBP) hijacks twist1 to inhibit vasculogenic mimicry in hepatocellular carcinoma; Authors - Lu-Ning Qin