Anticorps Monoclonal anti-Vitamin D binding protein

Vitamin D binding protein Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E4D10

N° de cat : 66175-1-PBS

Synonymes

GC, VDBP,GC, DBP, DBP/GC, DBP-maf

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Galerie de données de validation

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  WB analysis of human testis using 66175-1-Ig (same clone as 66175-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66175-1-Ig (Vitamin D binding protein antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66175-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66175-1-Ig (same clone as 66175-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66175-1-Ig (Vitamin D binding protein antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66175-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66175-1-Ig (same clone as 66175-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66175-1-Ig (Vitamin D binding protein antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66175-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66175-1-Ig (same clone as 66175-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66175-1-Ig (Vitamin D binding protein antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66175-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66175-1-Ig (same clone as 66175-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Vitamin D binding protein antibody (66175-1-Ig, Clone: 1E4D10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66175-1-PBS in a different storage buffer formulation.

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  FC experiment of U-937 using 66175-1-Ig (same clone as 66175-1-PBS)

  1X10^6 U-937 cells were intracellularly stained with 0.4 ug Anti-Human Vitamin D binding protein (66175-1-Ig, Clone:1E4D10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66175-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66175-1-PBS cible Vitamin D binding protein dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Vitamin D binding protein Protéine recombinante Ag9803
• Nom complet: group-specific component (vitamin D binding protein)
• Masse moléculaire calculée: 474 aa, 53 kDa
• Poids moléculaire observé: 52-58 kDa
• Numéro d’acquisition GenBank: BC057228
• Symbole du gène: Vitamin D binding protein
• Identification du gène (NCBI): 2638
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Vitamin D binding protein is a sparsely glycosylated serum protein responsible for highly specific binding and tissue-specific delivery of vitamin D and its metabolites. In addition, it is also an actin scavenger, and is the precursor to the immunomodulatory protein, Gc-MAF. Vitamin D binding protein has been proposed to have significant roles in C5a chemotaxis, osteoclast development and possibly in macrophage activation/recruitment.

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