Anticorps Polyclonal de lapin anti-WAPL

• Phare
• Validé par KD/KO

WAPL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, ChIP, ELISA

Conjugaison

Non conjugué

Publications(13)

Avis (1)

N° de cat : 16370-1-AP

Synonymes

WAPL/FOE, FOE, Wings apart-like protein homolog, WAPL cohesin release factor, WAPAL

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Galerie de données de validation

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  WB analysis using 16370-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16370-1-AP (WAPL; WAPAL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 16370-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16370-1-AP (WAPL; WAPAL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 16370-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16370-1-AP (WAPL; WAPAL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16370-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using WAPL; WAPAL antibody (16370-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules HepG2, cellules Jurkat
• Résultats positifs en IHC: tissu de muscle squelettique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IF: See 2 publications below
• ChIP: See 3 publications below

Informations sur le produit

16370-1-AP cible WAPL dans les applications de WB, IHC, IF/ICC, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WAPL Protéine recombinante Ag9280
• Nom complet: wings apart-like homolog (Drosophila)
• Masse moléculaire calculée: 142 kDa
• Poids moléculaire observé: 160-180 kDa
• Numéro d’acquisition GenBank: BC017393
• Symbole du gène: WAPL
• Identification du gène (NCBI): 23063
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The wings apart-like (WAPL, or WAPAL) protein was originally identified as a gene product that potentially regulates heterochromatin organization in Drosophila melanogaster. Human WAPL is a cohesin-binding protein that promotes sister-chromatid resolution in mitotic prophase. Overexpression of WAPL disturbs mitosis and cytokinesis, and contributes to tumor progression by induction of chromosomal instability. WAPAL has a predicted molecular weight of 140 kDa, however, as a result of its extensive phosphorylation it separates on a SDS gel as a band of approximately 160-180 kDa.

Publications

Species	Application	Title
human,mouse	WB,ChIP	Nat Genet; WAPL maintains a cohesin loading cycle to preserve cell-type-specific distal gene regulation.; Authors - Ning Qing Liu ; KO Validated
human	WB	Sci Adv; RNA polymerase II is required for spatial chromatin reorganization following exit from mitosis.; Authors - Shu Zhang
mouse	WB	J Clin Invest; Allogeneic T cell responses are regulated by a specific miRNA-mRNA network.; Authors - Sun Yaping Y
mouse	WB	Genome Biol; CTCF and cohesin promote focal detachment of DNA from the nuclear lamina; Authors - Tom van Schaik
human	WB	Curr Biol; Esco1 Acetylates Cohesin via a Mechanism Different from That of Esco2.; Authors - Masashi Minamino
mouse	WB,IF	Cell Rep; Cohesin Removal along the Chromosome Arms during the First Meiotic Division Depends on a NEK1-PP1γ-WAPL Axis in the Mouse.; Authors - Miguel A Brieño-Enríquez

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zhenmin (Verified Customer) (12-05-2019)

This antibody is very specific and works very well with immunohistochemical staining for formalin fixed and paraffin embedded tissue.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse Testis

WAPL Antibody Immunohistochemistry, validation (1:200 dilution) in Mouse Testis (Cat no:16370-1-AP)

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