Anticorps Recombinant de lapin anti-WAPL

WAPL Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242443C1

N° de cat : 84877-5-PBS

Synonymes

WAPL/FOE, FOE, 242443C1, WAPL cohesin release factor, WAPAL

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Galerie de données de validation

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  WB analysis using 84877-5-RR (same clone as 84877-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84877-5-RR (WAPL antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84877-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 84877-5-RR (same clone as 84877-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84877-5-RR (WAPL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84877-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 84877-5-RR (same clone as 84877-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84877-5-RR (WAPL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84877-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84877-5-RR (same clone as 84877-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using WAPL antibody (84877-5-RR, Clone: 242443C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84877-5-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84877-5-RR (same clone as 84877-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug WAPL Recombinant antibody (84877-5-RR, Clone:242443C1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84877-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84877-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84877-5-RR against Human WAPL were performed. The affinity constant is 0.23 nM.

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Informations sur le produit

84877-5-PBS cible WAPL dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: WAPL Protéine recombinante Ag9585
• Nom complet: wings apart-like homolog (Drosophila)
• Masse moléculaire calculée: 142 kDa
• Poids moléculaire observé: 160-180 kDa
• Numéro d’acquisition GenBank: BC017393
• Symbole du gène: WAPL
• Identification du gène (NCBI): 23063
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The wings apart-like (WAPL, or WAPAL) protein was originally identified as a gene product that potentially regulates heterochromatin organization in Drosophila melanogaster. Human WAPL is a cohesin-binding protein that promotes sister-chromatid resolution in mitotic prophase. Overexpression of WAPL disturbs mitosis and cytokinesis, and contributes to tumor progression by induction of chromosomal instability. WAPAL has a predicted molecular weight of 140 kDa, however, as a result of its extensive phosphorylation it separates on a SDS gel as a band of approximately 160-180 kDa.

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