Anticorps Polyclonal de lapin anti-WBP2NL

WBP2NL Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 22587-1-AP

Synonymes

PAWP, Postacrosomal sheath WW domain-binding protein, WW domain-binding protein 2-like



Applications testées

Résultats positifs en WBtissu testiculaire humain, tissu testiculaire de souris
Résultats positifs en IHCtissu testiculaire de souris, tissu ovarien de rat, tissu ovarien de souris, tissu testiculaire de rat
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochimie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

22587-1-AP cible WBP2NL dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéeHumain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène WBP2NL Protéine recombinante Ag18347
Nom complet WBP2 N-terminal like
Masse moléculaire calculée 309 aa, 32 kDa
Poids moléculaire observé 32 kDa
Numéro d’acquisition GenBankBC022546
Symbole du gène WBP2NL
Identification du gène (NCBI) 164684
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WBP2NL, also known as PAWP, is a protein found in the post-acrosomal region of mammalian spermatozoa. It was initially proposed as a sperm-borne oocyte-activating factor (SOAF) that triggers calcium oscillations essential for oocyte activation and embryo development. However, recent studies have shown mixed results regarding its role in fertilization. While some investigations suggest that PAWP can induce oocyte activation, others indicate that it may not be sufficient to compensate for the absence of other critical factors like PLCZ1. Additionally, altered WBP2NL expression has been observed in breast cancer tissues, hinting at its potential involvement in oncogenic regulation. (PMID: 24907910, PMID: 25417742, PMID: 28663133)

Protocole

Product Specific Protocols
WB protocol for WBP2NL antibody 22587-1-APDownload protocol
IHC protocol for WBP2NL antibody 22587-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Nat Commun

CEP128 is involved in spermatogenesis in humans and mice.

Authors - Xueguang Zhang
humanWB

Hum Mol Genet

Homozygous mutation of PLCZ1 leads to defective human oocyte activation and infertility that is not rescued by the WW binding protein PAWP.

Authors - Jessica Escoffier
WB,IF

Int J Mol Sci

Comprehensive Flow-Cytometric Quality Assessment of Ram Sperm Intended for Gene Banking Using Standard and Novel Fertility Biomarkers.

Authors - Jaromír Vašíček
mouseWB

Development

PLCζ is the physiological trigger of the Ca(2+) oscillations that induce embryogenesis in mammals but offspring can be conceived in its absence.

Authors - Alaa Hachem
humanWB,IF

Mol Hum Reprod

Functional disparity between human PAWP and PLCζ in the generation of Ca2+ oscillations for oocyte activation.

Authors - Michail Nomikos
humanWB

Mol Hum Reprod

Antigen unmasking enhances visualization efficacy of the oocyte activation factor, phospholipase C zeta, in mammalian sperm.

Authors - Junaid Kashir
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