Anticorps Polyclonal de lapin anti-WBSCR17

WBSCR17 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 21280-1-AP

Synonymes

EC:2.4.1.41, GalNAc T like protein 3, GalNAc-T-like protein 3, GALNT17, Polypeptide GalNAc transferase-like protein 3

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Galerie de données de validation

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  WB analysis of mouse brain using 21280-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21280-1-AP (WBSCR17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 21280-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21280-1-AP (WBSCR17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 21280-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21280-1-AP (WBSCR17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 21280-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21280-1-AP (WBSCR17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 21280-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21280-1-AP (WBSCR17 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 21280-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21280-1-AP (WBSCR17 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 21280-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21280-1-AP (WBSCR17 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 21280-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21280-1-AP (WBSCR17 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules A549, tissu cérébral humain, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu cérébral humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

21280-1-AP cible WBSCR17 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WBSCR17 Protéine recombinante Ag15792
• Nom complet: Williams-Beuren syndrome chromosome region 17
• Masse moléculaire calculée: 598 aa, 68 kDa
• Poids moléculaire observé: 70~90 kDa
• Numéro d’acquisition GenBank: BC069624
• Symbole du gène: WBSCR17
• Identification du gène (NCBI): 64409
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WBSCR17, also known as GALNT17, which encodes a brain-expressed N-acetylgalactosaminyl transferase (GalNAcT), is located at the distal edge of a region that is commonly deleted or duplicated in Williams Beuren Syndrome (WBS), a developmental disorder with motor and coordination problems, impaired visuospatial memory, and abnormal social interaction (PMID: 31554716). WBSCR17 loss-of-function has significant effects on cerebellar development, and is associated with phenotypes including developmental delay, deficits in motor coordination, reduced exploratory activity, and impaired social behavior (PMID: 22787146). With the calculated molecular mass of recombinant WBSCR17 being 68 kDa, the 70-90-kDa glycoproteins could also be detected due to post-translational modifications (PMID: 22787146).

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