Anticorps Polyclonal de lapin anti-WDFY4

WDFY4 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 17558-1-AP

Synonymes

C10orf64, KIAA1607, WDFY family member 4, WDFY4

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Galerie de données de validation

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  IHC staining of human tonsillitis using 17558-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17558-1-AP (WDFY4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 17558-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17558-1-AP (WDFY4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 17558-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17558-1-AP (WDFY4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 17558-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17558-1-AP (WDFY4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17558-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17558-1-AP (WDFY4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17558-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17558-1-AP (WDFY4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IHC: tissu d'amygdalite humain, tissu cérébral de souris, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

17558-1-AP cible WDFY4 dans les applications de IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WDFY4 Protéine recombinante Ag11720
• Nom complet: WDFY family member 4
• Masse moléculaire calculée: 354 kDa
• Poids moléculaire observé: ~70 kDa
• Numéro d’acquisition GenBank: BC032420
• Symbole du gène: WDFY4
• Identification du gène (NCBI): 57705
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WDFY4, also named as C10orf64 and KIAA1607, is a huge protein with unknown functions. WDFY4 is expressed predominantly in immune tissues such as lymph nodes, the spleen, the thymus and the tonsils. According to recent genome-wide association studies (GWAS), WDFY4 was introduced as novel predisposing genes for SLE (Systemic Lupus Erythematosus) which is a heterogeneous complex relapsing-remitting autoimmune disease. This antibody detects the C-terminal of WDFY4. The gene WDFY4 has some isoforms, 354 kDa, 73 kDa, 115 kDa, 353 kDa, 325 kDa( from UNIPROT) and 1992aa, 222 kDa; 3224aa, 358kDa; 624aa, 69 kDa; 1270aa, 143 kDa; 1887aa, 211 kDa (From NCBI).

Publications

Species	Application	Title
human	IHC	Front Immunol; New insights into markers for distinguishing neuroendocrine prostate cancer: evidence from single-cell analysis; Authors - Hailang Luo

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